雄激素对卵母细胞生长分化因子

山东大学学报(医学版)

雄激素对卵母细胞生长分化因子

陈子江

山东大学山东省立医院生殖医学中心，山东济南 250021

收稿日期:2006-04-05 修回日期:1900-01-01 出版日期:2006-06-24 发布日期:2006-06-24
通讯作者: 陈子江

CHEN Zi-jiang

Received:2006-04-05 Revised:1900-01-01 Online:2006-06-24 Published:2006-06-24
Contact: CHEN Zi-jiang

摘要/Abstract

摘要： 通过观察雄激素对小鼠卵母细胞生长分化因子9（growth differentiation factor9,GDF9）表达的影响，研究GDF9参与卵泡生长发育及其表达调控机制，探讨GDF9与多囊卵巢综合征(polycystic ovarian syndrome,PCOS)的关系。方法：以性成熟昆明小鼠为研究模型，体内实验分为实验组（注射丙酸睾丸酮）和对照组（注射等体积的生理盐水），每组各20只小鼠，采用原位杂交法检测卵巢组织GDF9 mRNA的表达水平。体外实验：胶原酶消化加机械法分离性成熟昆明小鼠卵泡进行体外培养，实验组培养液中加睾丸酮,对照组不加睾丸酮,72?h后收集卵泡，免疫组化检测卵母细胞GDF9的表达水平。结果：体内实验：实验组较对照组卵巢组织GDF9 mRNA表达水平降低, 差异有统计学意义（P＜0.05）；体外实验：实验组卵母细胞GDF9蛋白表达较对照组降低，差异有统计学意义（P＜0.05）。结论：雄激素减弱卵母细胞GDF9 mRNA和蛋白的表达强度；表达强度降低的GDF9可能与PCOS卵泡发育停滞有关

Abstract: To To investigate the effect of androgen on GDF9 expression both in vivo and in vitro. Methods： In vivo: In situ hybridization technique was used to measure the expression of GDF9 mRNA in mouse ovaries following once 2.5mg testosterone (experiment group) or NS (control group) every one day, 3 times, respectively. In vitro: Immunohistochemisty was used to detect the expression of GDF9 mRNA in mouse follicles 72 hours after cultured with (experiment group) or without （control group）testosterone. Results： In vivo: The expression of GDF9 mRNA was lower in experiment group than in control group (P＜0.05). In vitro: The expression of GDF9 protein in experiment group was significantly lower than that in control group (P＜0.05). Conclusion： Androgen may decrease the expression of GDF9 mRNA and protein, which is likely associated with the follicular development arrest.

Key words: Growth differentiation factor9, Polycystic ovarian syndrome, In suit hybridization, Immunohistochemistry

陈子江. 雄激素对卵母细胞生长分化因子[J]. 山东大学学报(医学版), 2006, 44(6): 549-552.

CHEN Zi-jiang. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(6): 549-552.

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