纳米TiO2联合Cu2+对斑马鱼胚胎发育毒性的影响

doi:10.6040/j.issn.1671-7554.0.2017.1170

山东大学学报 (医学版) ›› 2018, Vol. 56 ›› Issue (7): 33-38.doi: 10.6040/j.issn.1671-7554.0.2017.1170

• • 上一篇

纳米TiO₂联合Cu²⁺对斑马鱼胚胎发育毒性的影响

刘发生^1,2,韩建²,李莎¹,尚红园¹,马敏¹,王荣春²,张爱平¹,韩利文²

1.山西医科大学药学院, 山西太原 030001; 2.齐鲁工业大学(山东省科学院), 山东省科学院生物研究所, 山东省科学院药物筛选技术重点实验室, 山东济南 250014

发布日期:2022-09-27
通讯作者: 张爱平. E-mail: zhangapl@163.com 韩利文. E-mail: hanliwen08@126.com
基金资助:
山东省自然科学基金三院联合基金项目(ZR2015YL013)

Effect of nano titanium dioxide and copper ions on the developmental toxicity of zebrafish embryo

LIU Fasheng^1,2, HAN Jian², LI Sha¹, SHANG Hongyuan¹, MA Min¹, WANG Rongchun², ZHANG Aiping¹, HAN Liwen²

1. School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, Shanxi, China;
2. Biology Institute, Key Laboratory for Drug Screening Technology, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, Shandong, China

Published:2022-09-27

摘要/Abstract

摘要： 目的考察纳米TiO₂与Cu²⁺联合暴露对斑马鱼胚胎发育毒性的影响。方法采用AB系斑马鱼为动物模型,以斑马鱼胚胎在72 hpf孵化率、96 hpf死亡率及120 hpf畸形率为指标,评价纳米TiO₂联合Cu²⁺对斑马鱼胚胎发育的毒性作用。结果 (0.12~4)mg/L Cu²⁺对斑马鱼胚胎具有较强的毒性作用;(0~200)mg/L纳米TiO₂对斑马鱼胚胎发育无明显影响。纳米TiO₂与Cu²⁺联合暴露对斑马鱼胚胎有一定的发育毒性,但与单独Cu²⁺暴露相比,斑马鱼胚胎的死亡率、畸形率明显下降,孵化率明显上升。结论纳米TiO₂联合Cu²⁺在一定程度上降低了Cu²⁺对斑马鱼胚胎的发育毒性作用。

关键词: 纳米TiO₂, Cu²⁺, 联合暴露, 斑马鱼胚胎, 发育毒性

Abstract: Objective To investigate the effect of nano titanium dioxide(nano-TiO₂)combined with Cu²⁺ on the developmental toxicity of zebrafish embryos. Methods AB-line zebrafish was used for the animal model, the developmental toxicity of zebrafish embryos was evaluated by the hatching rate at 72 hours post fertilization(hpf), mortality rate at 96 hpf, and malformation rate at 120 hpf. Results Cu²⁺(0.12 - 4 mg/L)showed strong toxic effect on zebrafish embryos, while nano-TiO₂(0 - 200 mg/L)had no significant toxic effect on zebrafish embryos. The zebrafish embryos exposed to nano-TiO₂ and Cu²⁺ showed a degree of developmental toxicity, while the corresponding mortality rate and malformation rate were decreased and hatching rate was increased compared with those exposed to Cu²⁺. Conclusion Compared with Cu²⁺, the combination of nano-TiO₂ and Cu²⁺ shows lower development toxicity on zebrafish embryos.

Key words: Nano titanium dioxide, Cu²⁺, Combined exposure, Zebrafish embryos, Developmental toxicity

中图分类号:

X503.2

刘发生,韩建,李莎,尚红园,马敏,王荣春,张爱平,韩利文. 纳米TiO₂联合Cu²⁺对斑马鱼胚胎发育毒性的影响[J]. 山东大学学报 (医学版), 2018, 56(7): 33-38.

LIU Fasheng, HAN Jian, LI Sha, SHANG Hongyuan, MA Min, WANG Rongchun, ZHANG Aiping, HAN Liwen. Effect of nano titanium dioxide and copper ions on the developmental toxicity of zebrafish embryo[J]. Journal of Shandong University (Health Sciences), 2018, 56(7): 33-38.

参考文献

[1] 王狄,李锋民,熊治廷,等.铜的植物毒性与植物蓄积的关系[J].土壤与环境, 2000, 9(2): 146-148. WANG Di, LI Fengmin, XIONG Zhiting, et al. Relationship between coppers toxicity and phytoaccumulation[J]. Soil and Environmental Sciences, 2000, 9(2): 146-148.
[2] 万小华.铜过量导致的肝损伤及姜黄素的保护作用[D].武汉:华中科技大学, 2007.
[3] 阮喜云,钟士江,刘庆勇,等.铜过量的表现[J].国外医学(医学地理分册), 1999, 20(4): 157-159.
[4] Yin ZF, Wu L, Yang HG, et al. Recent progress in biomedical applications of titanium dioxide[J].Phys Chem Chem Phys, 2013, 15(14): 4844-4858.
[5] Jiang ZF, Zhu CZ, Wan WM, et al. Constructing graphite-like carbon nitride modified hierarchical yolk-shell TiO₂ spheres for water pollution treatment and hydrogen production[J]. J Mater Chem A, 2016, 4(5): 1806-1818.
[6] Villatte G, Massard C, Descamps S, et al. Photoactive TiO₂ antibacterial coating on surgical external fixation pins for clinical application[J]. Int J Nanomedicine, 2015, 10(1): 3367-3375.
[7] Strähle U, Scholz S, Geisler R, et al. Zebrafish embryos as an alternative to animal experiments—a commentary on the definition of the onset of protected life stages in animal welfare regulations[J]. Reprod Toxicol, 2012, 33(2): 128-132.
[8] 彭蕴茹,韦英杰,丁永芳,等.基于斑马鱼模型的药物毒性研究进展与中药毒性研究新策略[J].中草药, 2017, 48(1): 17-30. PENG Yunru, WEI Yingjie, DING Yongfang, et al. Development of drug toxicity and novel strategy for toxicity of Chinese materia medica based on zebrafish model[J]. Chinese Traditional and Herbal Drugs, 2017, 48(1): 17-30.
[9] Parichy DM, Elizondo MR, Mills MG, et al. Normal table of postembryonic zebrafish development: staging by externally visible anatomy of the living fish[J]. Dev Dynam, 2009, 238(12): 2975-3015.
[10] Lee O, Green JM, Tyler CR. Transgenic fish systems and their application in ecotoxicology[J]. Crit Rev Toxicol, 2014, 45(2): 124-141.
[11] Scholz S, Fischer S, Gündel U, et al. The zebrafish embryo model in environmental risk assessment-applications beyond acute toxicity testing[J]. Environ Sci Pollut R, 2008, 15(5): 394-404.
[12] Chakraborty C, Sharma AR, Sharma G, et al. Zebrafish: a complete animal model to enumerate the nanoparticle toxicity[J]. J Nanobiotechnol, 2016, 14(1): 65-78.
[13] Shin JT, Fishman MC. From zebrafish to human: Modular medical models[J]. Annu Rev Genom Hum G, 2002, 3(1): 311-340.
[14] Kimmel CB, Ballard WW, Kimmel SR, et al. Stages of embryonic development of the zebrafish[J]. Dev Dynam, 1995, 203(3): 253-310.
[15] Villamizar N, Ribas L, Piferrer F, et al. Impact of daily thermocycles on hatching rhythms, larval performance and sex differentiation of zebrafish[J]. PLoS One, 2012, 7(12): e52153. doi: 10.1371/journal.pone.0052153.
[16] Handy RD, Owen R, Valsami-Jones E. The ecotoxicology of nanoparticles and nanomaterials: current status, knowledge gaps, challenges, and future needs[J]. Ecotoxicology, 2008, 17(5): 315-325.
[17] Ju-Nam Y, Lead JR. Manufactured nanoparticles: an overview of their chemistry, interactions and potential environmental implications[J]. Sci Total Environ, 2008, 400(1-3): 396-414.
[18] 张守申. 二氧化钛纳米与重金属Cd离子相互作用降低Cd²⁺对秀丽隐杆线虫的毒性[D].济南:山东大学, 2014.
[19] Zhang X, Sun H, Zhang Z, et al. Enhanced bioaccumulation of cadmium in carp in the presence of titanium dioxide nanoparticles[J]. Chemosphere, 2007, 67(1):160-166.
[20] Sun H, Zhang X, Zhang Z, et al. Influence of titanium dioxide nanoparticles on speciation and bioavailability of arsenite[J]. Environ Pollut, 2009, 157(4): 1165-1170.
[21] Tang Y, Li S, Qiao J, et al. Synergistic effects of nano-sized titanium dioxide and zinc on the photosynthetic capacity and survival of Anabaena sp[J]. Int J Mol Sci, 2013, 14(7): 14395-14407.
[22] Rosenfeldt RR, Seitz F, Schulz R, et al. Heavy metal uptake and toxicity in the presence of titanium dioxide nanoparticles: a factorial approach using Daphnia magna[J]. Environ Sci Technol, 2014, 48(12): 6965-6972.
[23] 蔡文超,区又君.重金属离子铜对鱼类早期发育阶段的毒性[J].南方水产, 2009, 5(5): 75-79. CAI Wenchao, OU Youjun. Toxicity of Cu²⁺ to fish during early developmental stages: a review[J]. South China Fisheries Science, 2009, 5(5): 75-79.
[24] 尤宏,吕丽娜,赵雪松,等.几种纳米材料对斑马鱼孵化和致畸效应的影响[J].哈尔滨工业大学学报, 2013, 45(12): 48-52. YOU Hong, LÜ Lina, ZHAO Xuesong, et al. Effects of nanomaterials on the hatching and malformation rate of zebrafish embryos[J]. Journal of Harbin Institute of Technology, 2013, 45(12): 48-52.
[25] Bar-Ilan O, Louis KM, Yang SP, et al. Titanium dioxide nanoparticles produce phototoxicity in the developing zebrafish[J]. Nanotoxicology, 2012, 6(6): 670-679.
[26] Clemente Z, Castro VL, Moura MA, et al. Toxicity assessment of TiO₂ nanoparticles in zebrafish embryos under different exposure conditions[J]. Aquat Toxicol, 2014, 147(2): 129-139.

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纳米TiO₂联合Cu²⁺对斑马鱼胚胎发育毒性的影响

Effect of nano titanium dioxide and copper ions on the developmental toxicity of zebrafish embryo

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