丙戊酸对卵巢癌裸鼠移植瘤血管形成的影响

山东大学学报(医学版)

丙戊酸对卵巢癌裸鼠移植瘤血管形成的影响

杨高原，荣风年，周婷

山东大学医学院山东省千佛山医院妇产科，济南 250014

收稿日期:2008-06-02 修回日期:1900-01-01 出版日期:2008-11-16 发布日期:2008-11-16
通讯作者: 荣风年

Effect of valproic acid on angiogenesis of human ovarian cancer in nude mice

YANG Gao-yuan, RONG Feng-nian, ZHOU Ting

Department of Gynecology and Obstetrics, Shandong Qianfoshan Hospital, School of Clinical Medicine, Shandong University, Jinan 250014, China

Received:2008-06-02 Revised:1900-01-01 Online:2008-11-16 Published:2008-11-16
Contact: RONG Feng-nian

摘要/Abstract

摘要： 目的探讨丙戊酸(VPA）对卵巢癌裸鼠移植瘤血管形成的影响。方法建立人卵巢癌裸鼠皮下转移瘤模型，将24只裸小鼠随机分为对照组、VPA组、顺铂（DDP）组、VPA+DDP 4组，每组6只。观察并记录各组裸鼠及其皮下移植瘤的生长情况，绘制肿瘤生长曲线、计算肿瘤体积、肿瘤生长抑制率。采用免疫组化SP法检测各组裸鼠皮下移植瘤中CD105 和sFlk-1的表达水平。结果① 治疗组裸鼠移植瘤的体积较对照组明显变小；② 各治疗组肿瘤组织的CD105表达较对照组均明显下降(P＜0.05)，sFlk-1表达较对照组均明显增高(P＜0.05)。结论VPA对卵巢癌裸鼠移植瘤有明显抑制作用，其作用机理可能与抑制肿瘤血管形成有关，与DDP联合应用其抑瘤作用增强。

关键词: 小鼠, 裸, 顺铂, 卵巢肿瘤, 丙戊酸

Abstract: To investigate the inhibitory effect of valproic acid（VPA）on angiogenesis of human ovarian cancer in nude mice. MethodsHuman ovarian cancer models were established by subcutaneous transplantation in nude mice, and then they were divided into 4 groups: the control group, the VPA group, the cisplatin (DDP) group and the VPA+DDP group. The anti-tumor efficacy was evaluated by determination of tumor volume, tumor growth and relative tumor curative rate. Also expression levels of CD105 and sFlk-1 were determined by immunocytochemistry (IC). Results① Tumor volumes in the experimental groups were smaller than those in the control group; ② Expression levels of CD105 in the experimental groups were significantly higher than those in the control group(P＜0.05); and ③ Expression levels of sFlk-1 in the experimental groups were significantly higher than those in the control group(P＜0.05). ConclusionsVPA can inhibit the growth of ovarian cancer by decreasing the expression of CD105 and increasing the expression of sFlk-1 levels.

Key words: Valproic acid, Cisplatin, Ovarian neoplasms, Nude mice

中图分类号:

R737.31

杨高原,荣风年,周婷. 丙戊酸对卵巢癌裸鼠移植瘤血管形成的影响[J]. 山东大学学报(医学版), 2008, 46(11): 1034-1036.

YANG Gao-yuan,RONG Feng-nian,ZHOU Ting. Effect of valproic acid on angiogenesis of human ovarian cancer in nude mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(11): 1034-1036.

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