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山东大学学报(医学版) ›› 2016, Vol. 54 ›› Issue (3): 5-8.doi: 10.6040/j.issn.1671-7554.0.2015.409

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吸氧浓度对心肺复苏大鼠S100β蛋白和神经元特异性烯醇化酶表达的影响

王立敏1,王旭东2   

  1. 辽宁医学院航天中心医院 1.研究生培养基地;2.急救部, 北京 100049
  • 收稿日期:2015-04-23 出版日期:2016-03-10 发布日期:2016-03-10
  • 通讯作者: 王旭东. E-mail:wjrd6@sina.com E-mail:wjrd6@sina.com

Effects of oxygen with different concentrations on the expressions of S100β protein and neuron-specific enolase after cardiopulmonary resuscitation in rats

WANG Limin1, WANG Xudong2   

  1. 1. Postgraduate Training Base;
    2. Department of Emergency, Aerospace Central Hospital of Liaoning Medical University, Beijing 100049, China
  • Received:2015-04-23 Online:2016-03-10 Published:2016-03-10

摘要: 目的 探讨不同给氧浓度对大鼠心肺复苏(CPR)结局的影响。 方法 选取成年雄性SD大鼠32只,按随机原则分成对照组与复苏组(包括21%氧浓度组、50%氧浓度组及100%氧浓度组),每组8只。对照组仅行麻醉、气管插管和血管穿刺。复苏组建立大鼠心脏骤停(CA)/CPR模型,分别给予21%、50%和100%浓度的氧气。采用ELISA法检测每组复苏成功后0.5、3、6 h大鼠血清S100β蛋白、神经元特异性烯醇化酶(NSE)水平。 结果 与对照组相比,复苏组大鼠血清S100β蛋白水平均于复苏成功后逐渐升高(P<0.05),并于复苏后3 h达到峰值;复苏组大鼠NSE水平随时间增加逐渐升高(P<0.05)。复苏组组间对比:21%氧浓度组大鼠血清S100β蛋白、NSE水平升高最为明显,100%氧浓度组次之,50%氧浓度组升高程度最低,差异有统计学意义(P<0.05)。 结论 给予50%浓度氧气较100%、21%浓度氧气更能降低CPR大鼠血清S100β蛋白和NSE水平,并减轻CPR后的继发性脑损伤,从而改善脑功能。

关键词: 神经元特异性烯醇化酶, 心脏骤停, S100β蛋白, 心肺复苏

Abstract: Objective To investigate the effects of oxygen with different concentrations on the outcome of cardiopulmonary resuscitation(CPR)in rats. Methods Thirty-two SD rats were randomly divided into the control group and resuscitation group, which was subdivided into 21% oxygen concentration group, 50% oxygen concentration group and 100% oxygen concentration group, with 8 in each group. Anesthesia, tracheal intubation, and vascular puncturation were performed for the control group. Other groups were given 21%, 50% and 100% oxygen respectively. ELISA assay was used to detect the levels of of serum S100β protein and neuron-specific enolase(NES)at 0.5, 3, and 6 h after successful CPR. Results Compared with the control group, the level of serum S100β protein in resuscitation group was higher(P<0.05)and reached the peak at 3h time point, while the level of serum NSE rose stably with the increase of time(P<0.05). After a successful resuscitation, the risen levels of S100β protein and NSE were as follows: 21% oxygen concentration group > 100% oxygen concentration group > 50% oxygen concentration group(P<0.05). Conclusion Compared with 21% and 100% oxygen, 50% oxygen inhalation can decrease the levels of serum S100β protein and NSE after a successful CPR, minimize secondary brain injury and thus improve the brain functionality.

Key words: Neuron-specific enolase, Cardiopulmonary resuscitation, S100β protein, Cardiac arrest

中图分类号: 

  • R459.7
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