吸氧浓度对心肺复苏大鼠S100β蛋白和神经元特异性烯醇化酶表达的影响

doi:10.6040/j.issn.1671-7554.0.2015.409

摘要/Abstract

摘要： 目的探讨不同给氧浓度对大鼠心肺复苏(CPR)结局的影响。方法选取成年雄性SD大鼠32只,按随机原则分成对照组与复苏组(包括21%氧浓度组、50%氧浓度组及100%氧浓度组),每组8只。对照组仅行麻醉、气管插管和血管穿刺。复苏组建立大鼠心脏骤停(CA)/CPR模型,分别给予21%、50%和100%浓度的氧气。采用ELISA法检测每组复苏成功后0.5、3、6 h大鼠血清S100β蛋白、神经元特异性烯醇化酶(NSE)水平。结果与对照组相比,复苏组大鼠血清S100β蛋白水平均于复苏成功后逐渐升高(P<0.05),并于复苏后3 h达到峰值;复苏组大鼠NSE水平随时间增加逐渐升高(P<0.05)。复苏组组间对比:21%氧浓度组大鼠血清S100β蛋白、NSE水平升高最为明显,100%氧浓度组次之,50%氧浓度组升高程度最低,差异有统计学意义(P<0.05)。结论给予50%浓度氧气较100%、21%浓度氧气更能降低CPR大鼠血清S100β蛋白和NSE水平,并减轻CPR后的继发性脑损伤,从而改善脑功能。

关键词: 神经元特异性烯醇化酶, 心脏骤停, S100β蛋白, 心肺复苏

Abstract: Objective To investigate the effects of oxygen with different concentrations on the outcome of cardiopulmonary resuscitation(CPR)in rats. Methods Thirty-two SD rats were randomly divided into the control group and resuscitation group, which was subdivided into 21% oxygen concentration group, 50% oxygen concentration group and 100% oxygen concentration group, with 8 in each group. Anesthesia, tracheal intubation, and vascular puncturation were performed for the control group. Other groups were given 21%, 50% and 100% oxygen respectively. ELISA assay was used to detect the levels of of serum S100β protein and neuron-specific enolase(NES)at 0.5, 3, and 6 h after successful CPR. Results Compared with the control group, the level of serum S100β protein in resuscitation group was higher(P<0.05)and reached the peak at 3h time point, while the level of serum NSE rose stably with the increase of time(P<0.05). After a successful resuscitation, the risen levels of S100β protein and NSE were as follows: 21% oxygen concentration group > 100% oxygen concentration group > 50% oxygen concentration group(P<0.05). Conclusion Compared with 21% and 100% oxygen, 50% oxygen inhalation can decrease the levels of serum S100β protein and NSE after a successful CPR, minimize secondary brain injury and thus improve the brain functionality.

Key words: Neuron-specific enolase, Cardiopulmonary resuscitation, S100β protein, Cardiac arrest

中图分类号:

R459.7

王立敏,王旭东. 吸氧浓度对心肺复苏大鼠S100β蛋白和神经元特异性烯醇化酶表达的影响[J]. 山东大学学报（医学版）, 2016, 54(3): 5-8.

WANG Limin, WANG Xudong. Effects of oxygen with different concentrations on the expressions of S100β protein and neuron-specific enolase after cardiopulmonary resuscitation in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 5-8.

参考文献

[1] Xu J, Sun S, Lu X, et al. Remote ischemic pre- and postconditioning improve postresuscitation myocardial and cerebral function in a rat model of cardiac arrest and resuscitation[J]. Crit Care Med, 2015, 43(1): e12-e18.
[2] Miyata K, Mikami T, Asai Y, et al. Subarachnoid hemorrhage after resuscitation from out-of-hospital cardiac arrest[J]. J Stroke Cerebrovasc Dis, 2014, 23(3): 446-452.
[3] 谷兴华,张供,张希全,等.血清蛋白B亚型和神经元特异性烯醇化酶对早期判断体外循环术后脑损伤的意义[J].中华医学杂志, 2007, 87(14): 975-977. GU XingHua, ZHANG Gong, ZHANG Xiquan, et al. Clinical values of detection of serum levels of S100B and NSE in diagnosis of brain injuries at early period after cardiopulmonary bypass[J]. Natl Med J China, 2007, 87(14): 975-977.
[4] Sramko M, Peichl P, Wichterle D, et al. A novel biomarker-based approach for the detection of asymptomatic brain injury during catheter ablation of atrial fibrillation[J]. J Cardiovasc Electrophysiol, 2014, 25(4): 349-354.
[5] Martínez-Morillo E, García Hernández P, Begcevic I, et al. Identification of novel biomarkers of brain damage in patients with hemorrhagic stroke by integrating bioinformatics and mass spectrometry-based proteomics[J]. J Proteome Res, 2014, 13(2): 969-981.
[6] Rohlwink UK, Figaji AA. Biomarkers of brain injury in cerebral infections[J]. Clin Chem, 2014, 60(6): 823-834.
[7] Thel MC, OConnor CM. Cardiopulmonary resuscitation: historical perspective to recent investigations[J]. Am Heart J, 1999, 137(1): 39-48.
[8] Peberdy MA, Kaye W, Ornato JP, et al. Cardiopulmonary resuscitation of adults in the hospital: a report of 14720 cardiac arrests from the National Registry of Cardiopulmonary Resuscitation[J]. Resuscitation, 2003, 58(3): 297-308.
[9] 沈洪,王士雯. 2003:中国心肺复苏的关注问题[J].中国危重病急救医学, 2003, 15(5): 262-264.
[10] 李媛媛,张楠,徐谧山,等.新生小鼠缺氧缺血脑组织基质细胞衍生因子1α的表达及其作用[J].山东大学学报(医学版), 2015, 53(10): 26-31. LI Yuanyuan, ZHANG Nan, XU Mishan, et al. Expressing of stromal cell derived factor-1α and its effects in hypoxic-ischemic brain tissue of neonatal mice[J]. Journal of Shandong University(Health Science), 2015, 53(10): 26-31.
[11] Wheeler KT, Payne V, DAgostino RB Jr, et al. Impact of breathing 100% oxygen on radiation-induced cognitive impairment[J]. Radiat Res, 2014, 182(5): 580-585.
[12] 孙斌,缪珀,任静,等.空气及纯氧对新生儿复苏效果的Meta分析[J].中国血液流变学杂志, 2014, 24(1): 110-114. SUN Bin, MIAO Po, REN Jing, et al. Effect of air and 100% oxygen resuscitation of newborn: a Meta-analysis[J]. Chinese Journal of Hemorheology, 2014, 24(1): 110-114.
[13] 陈素艳,高峰,张清勇,等.不同浓度常压氧对大鼠脑缺血再灌注损伤早期的保护作用[J].中华实验外科杂志, 2014, 31(1): 111-113. CHEN Suyan, GAO Feng, ZHANG Qingyong, et al. Protective effect of different concentrations of normobaric oxygen in early cerebral ischemic-reperfusion injury rat model[J]. Chin J Exp Surg, 2014, 31(1): 111-113.
[14] Clark SD, Saker F, Schneeberger MT, et al. Administration of 100% oxygen does not hasten resolution of symptomatic spontaneous pneumothorax in neonates[J]. J Perinatol, 2014, 34(7): 528-531.
[15] 朱佳骏,姜舟,吴明远.空气或纯氧复苏宫内窒息新生大鼠血气和大脑SOD水平的比较[J].中华急诊医学杂志, 2011, 20(1): 48-51. ZHU Jiajun, JIANG Zhou, WU Mingyuan. Effects of resuscitation with air or oxygen on blood gas and cerebral SOD concentration in neonatal rats with intrauterine asphyxia[J]. Chin J Emerg Med, 2011, 20(1): 48-51.
[16] Solberg R, Enot D, Deigner HP, et al. Metabolomic analyses of plasma reveals new insights into asphyxia and resuscitation in pigs[J]. PLoS One, 2010, 5(3): e9606.
[17] 蒋红斌,黎惟广.不同氧浓度复苏囊正压呼吸在早产儿原发性呼吸暂停中的应用[J].中国基层医药, 2011, 18(18): 2540-2541.
[18] 王珂,张振香,陈素艳,等.45%浓度氧对大鼠缺血再灌注损伤的实验研究[J].中国实用神经疾病杂志, 2013, 16(13): 50-51.
[19] 李神清,熊敏,孙新飞.血清BNP和S100蛋白在心肺复苏后脑损伤判断中的作用[J].国际检验医学杂志, 2014, 35(16): 2254-2256.
[20] Žurek J, Fedora M. The usefulness of S100B, NSE, GFAP, NF-H, secretagogin and Hsp70 as a predictive biomarker of outcome in children with traumatic brain injury[J]. Acta Neurochir(Wien), 2012, 154(1): 93-103.
[21] Larsson IM, Wallin E, Kristofferzon ML, et al. Post-cardiac arrest serum levels of glial fibrillary acidic protein for predicting neurological outcome[J]. Resuscitation, 2014, 85(12): 1654-1661.

相关文章 12

[1]	梁丽宁,钟霞,季宪飞,胡浩然,朱芳芳,陈吉彬,陈茜茜,商德亚. 尼可地尔后适应对猪心脏骤停后心脑肾缺血再灌注损伤的保护作用[J]. 山东大学学报（医学版）, 2017, 55(10): 46-51.
[2]	于玉娟,高成杰,王晓鹏,李波. 帕瑞昔布钠对脑肿瘤切除术患者炎症反应的影响及脑保护作用[J]. 山东大学学报（医学版）, 2016, 54(6): 65-68.
[3]	臧丽娇,仇杰,庄根苗,安丽. 血清S100B蛋白、神经元特异性烯醇化酶与新生儿低血糖脑损伤的相关性[J]. 山东大学学报（医学版）, 2016, 54(4): 51-54.
[4]	张志强,吴艳华,王茂水,王新锋,汪运山. 联合检测血清PCT和NSE对小细胞肺癌的诊断价值[J]. 山东大学学报（医学版）, 2016, 54(11): 36-39.
[5]	付海燕, 胡占升, 杜红阳, 李潮, 包翠芬. 地黄多糖对过表达Notch1(NICD)大鼠骨髓间充质干细胞诱导分化及增殖的影响[J]. 山东大学学报（医学版）, 2015, 53(1): 34-40.
[6]	吴平. 哌拉西林舒巴坦钠(4∶1)过敏致哮喘患儿心脏骤停1例[J]. 山东大学学报(医学版), 2013, 51(06): 110-110.
[7]	王伯松，钟敬泉，李景莎，曾绮娴，刘红臻，苏国英，刘东林，张运. 连续电除颤与单次电除颤在长时程心室颤动中的疗效对比研究[J]. 山东大学学报(医学版), 2011, 49(3): 37-.
[8]	王伯松，钟敬泉，李景莎，曾绮娴，刘红臻，苏国英，刘东林，张运. 连续电除颤与单次电除颤在长时程心室颤动中的疗效对比研究[J]. 山东大学学报(医学版), 2011, 49(3): 37-.
[9]	李景莎，钟敬泉，刘红臻，曾绮娴，苏国英，孟祥林，刘东林，张运. 室颤时冠脉灌注压和室颤波形对自主循环恢复的预测研究[J]. 山东大学学报(医学版), 2011, 49(2): 54-.
[10]	孟祥林,钟敬泉,苏国英,曾绮娴,刘红臻,刘东林,王远龙,李景莎,张运. 两种剂量肾上腺素在家猪长时程室颤心肺复苏中的疗效比较[J]. 山东大学学报(医学版), 2010, 48(4): 32-35.
[11]	上官红1，肖伟1，董亮1，李玉1，田辉2，郑天郢3. 血清CYFRA21-1、NSE和CEA在肺癌诊断和评估预后中的临床价值[J]. 山东大学学报(医学版), 2010, 48(12): 134-137.
[12]	钟敬泉,Paul Dorian,高海青,张薇,黎莉,钟明,葛志明,张梅,季晓平,王荣,蒋桂花,张运. Oxylator和阻抗阈值瓣膜对猪心肺复苏时冠脉灌注压的影响[J]. 山东大学学报(医学版), 2007, 45(4): 343-347.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed