肝癌细胞对人成熟树突状细胞影响的基因芯片分析

doi:10.6040/j.issn.1671-7554.0.2015.291

摘要/Abstract

摘要： 目的检测并分析肝癌细胞对人成熟树突状细胞(mDCs)基因转录水平的影响。方法利用免疫磁珠法分选出新鲜人外周血单核细胞,在体外经重组GM-CSF、IL-4和TNF-α诱导,培养并分化为mDCs。与Bel7402肝癌细胞共培养的mDCs为共培养组,正常培养的mDCs为对照组。共培养48 h后,Trizol法抽提总RNA,利用人树突状细胞基因芯片检测mDCs的基因在转录水平的变化表达。结果差异表达的基因共有663个(与对照组相比,变化表达倍数≥2),其中上调290个,下调373个。这些基因的功能主要涉及信号转导、细胞代谢和细胞周期等。结论肝癌细胞能在转录水平影响mDCs中多个基因的表达,这些基因控制并影响着mDCs的功能、增殖、分化和凋亡。

关键词: 树突状细胞, 肝肿瘤, 肿瘤微环境, 基因芯片

Abstract: Objective To detect the effects of hepatocellular carcinoma cells on the transcription of functional genes in maturate dendritic cells(mDCs). Methods Peripheral blood mononuclear cells(PBMCs)were enriched from freshly harvested whole blood by immunomagnetic beads. MDCs were induced by GM-CSF, IL-4 and TNF-α in vitro. Then, mDCs were cultured with(co-cultured group)or without(control group)hepatoma cells. Forty-eight hours later, total RNA of mDCs was collected and the expressions of functional genes were analyzed by using human dendritic cells gene array. Results There were 663 genes significantly regulated(compared with control group, fold changes≥ 2)in co-cultured group, of which 290 were up-regulated and 373 were down-regulated. These genes were associated with the functions of signal transduction, cell metabolism, cell cycle, and so on. Conclusion Hepatocellular carcinoma cells affect the transcription of some functional genes in mDCs, which thus affects its functions, proliferation, differentiation and apoptosis.

Key words: Tumor microenvironment, Microarrary, Liver neoplasms, Dendritic cells

中图分类号:

R318.04

杨晖,董蓉,薛慧,胡祖权,曾柱. 肝癌细胞对人成熟树突状细胞影响的基因芯片分析[J]. 山东大学学报（医学版）, 2016, 54(1): 1-6.

YANG Hui, DONG Rong, XUE Hui, HU Zuquan, ZENG Zhu. Gene microarray analysis of mature dendritic cells cultured with hepatocellular carcinoma cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(1): 1-6.

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