胃癌患者腹腔灌洗液CK19 mRNA、CEA mRNA的检测及其临床意义

山东大学学报(医学版)

胃癌患者腹腔灌洗液CK19 mRNA、CEA mRNA的检测及其临床意义

许德国，王潍博

山东大学附属省立医院肿瘤中心，济南 250021

收稿日期:2007-11-25 修回日期:1900-01-01 出版日期:2008-06-16 发布日期:2008-06-16
通讯作者: 王潍博

Expression of CK19 mRNA and CEA mRNA in peritoneal cavity washes of patients with gastric carcinoma and its clinical significance

XU De-guo， WANG Wei-bo

Department of Tumor Treatment Center, Provincial Hospital　 Affiliated to Shandong University, Jinan 250021, China

Received:2007-11-25 Revised:1900-01-01 Online:2008-06-16 Published:2008-06-16
Contact: WANG Wei-bo

摘要/Abstract

摘要： 目的观察采用巢式逆转录聚合酶链反应（RT-PCR）检测胃癌患者腹腔冲洗液中细胞角蛋白19（CK19）mRNA和癌胚抗原（CEA）mRNA的临床价值。方法收集67例胃癌患者和8例胃良性病变患者腹腔冲洗液或腹水。常规行H-E染色细胞学检查，采用巢式RT-PCR方法检测腹腔冲洗液中CK19 mRNA和CEA mRNA的表达，并以胃癌细胞SGC7901为阳性对照。结果67例胃癌患者腹腔冲洗液中CK19 mRNA的阳性表达率为58.2％（39/67）；CEA mRNA的阳性表达率为53.7％（36/67）；CK19 mRNA和CEA mRNA的联合检测阳性率为67.2％（45/67），皆高于细胞病理学检查的19.4％（13/67），而13例细胞病理学检查阳性患者的CK19 mRNA、CEA mRNA检测结果均为阳性。CK19 mRNA、CEA mRNA阳性率与肿瘤胃壁浆膜受侵、淋巴结转移和胃癌分期明显相关（P＜0.05）。结论采用巢式RT-PCR方法检测胃癌患者腹腔冲洗液中CK19 mRNA、CEA mRNA的表达敏感性高，对于早期诊断胃癌患者腹腔种植转移有一定临床价值。

关键词: 耾胃皿Ｄ縛胆觞蛒盋}1Ｙ瘛职拚厗?

Abstract: To explore the effect of CK19 mRNA and CEA mRNA expressions in peritoneal cavity washes for gastric cancer. MethodsPeritoneal irrigating solution or abdominal dropsy was collected from 67 patients with advanced gastric cancer and 8 patients with benign lesion on the stomach. Nested RT-PCR and cytopathological examination were used to determine the CK19 mRNA and CEA mRNA expressions. Meanwhile, the gastric cancer cell line SGC7901 served as a positive control. ResultsPositive expression of CK19 mRNA was found in 39 patients (58.2%), of CEA mRNA was in 36 patients(53.7%), and of CK19 mRNA and CEA mRNA was in 45 patients(67.2%) by nested RT-PCR. However, positive expression of both CK19 mRNA and CEA mRNA was found in 13 patients(19.4%) by cytopathological examination. There was a close correlation between CK19 mRNA and CEA-mRNA expressions and pathology and TNM staging in gastric cancer patients. ConclusionsNested RT-PCR is a sensitive method for CK19 mRNA and CEA mRNA expression in gastric cancer, and it is promising as a valuable method for predicting celiac implantation metastasis in gastric cancer.

Key words: Gastric neoplasm, Cytokeratin19, Carcinoembryonic antigen

中图分类号:

R735.2

许德国,王潍博. 胃癌患者腹腔灌洗液CK19 mRNA、CEA mRNA的检测及其临床意义[J]. 山东大学学报(医学版), 2008, 46(6): 600-603.

XU De-guo,WANG Wei-bo. Expression of CK19 mRNA and CEA mRNA in peritoneal cavity washes of patients with gastric carcinoma and its clinical significance[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(6): 600-603.

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