不明原因早期复发性自然流产患者蜕膜组织CD158受体的表达

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (8): 103-107.

不明原因早期复发性自然流产患者蜕膜组织CD158受体的表达

王珊1，陈子江2，许成岩2，宋辉3，赵跃然4，傅艺冰1

1.山东大学附属省立医院妇产科，济南 250021； 2.山东大学附属省立医院生殖医学中心，济南 250021；
3.山东大学附属济南市中心医院心内科，济南 250013； 4.山东大学附属省立医院中心实验室，济南 250021

收稿日期:2012-03-07 出版日期:2012-08-10 发布日期:2012-08-10
通讯作者: 傅艺冰(1972- )，女，博士，副主任医师，主要从事妇产科常见病研究。 E-mail：fu-yibing@163.com
作者简介:王珊(1978- )，女，主治医师，博士，主要从事复发性流产研究
基金资助:
国家自然科学基金(81100447，81170590)；山东省自然科学基金(ZR2009CQ005，ZR2011HM040)；山东省科技发展计划(2009GG100020)

Expression of CD158 in deciduas of women with early unexplained
recurrent spontaneous abortion

WANG Shan1， CHEN Zi-jiang2， XU Cheng-yan2， SONG Hui3， ZHAO Yue-ran4， FU Yi-bing1

1. Department of Obstetrics and Gynecology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China；
2. Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China；
3. Department of Cardiology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China；
4. Department of Central Laboratory， Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2012-03-07 Online:2012-08-10 Published:2012-08-10

摘要/Abstract

摘要：

目的探讨不明原因早期复发性自然流产(RSA)患者和正常对照组早孕子宫蜕膜组织中杀伤细胞免疫球蛋白样受体(KIR)CD158表达的特征。方法选择2009年1月至2010年6月就诊的RSA患者和同期计划生育门诊因计划外孕行人工流产术者各30例，并根据孕周分组，流式细胞术检测蜕膜组织细胞表面标记，比较RSA患者和对照者CD56、CD16、CD158a(KIR2DL1/S1)、CD158b(KIR2DL2/S2)表达的变化规律。结果 ① RSA患者CD56+细胞数显著少于对照者(P=0.007)，CD56+/CD16-细胞较对照组降低(P＜0.05)；② RSA患者CD158a在CD56+/CD16-细胞表面的表达显著降低(P=0.026)；③ 按孕周分组分析显示，孕7~10周患者CD56+/CD16-细胞表面表达CD158a较同期对照者显著降低(P=0.010)；④ 正常早孕蜕膜组织CD158a、CD158b与CD56表达呈显著正相关(P＜0.01)。结论早孕蜕膜组织CD56+/CD16-细胞减少可能为不明原因自然流产的发病机制之一。 CD56+/CD16-细胞表面CD158a表达下降与不明原因自然流产有关，且其变化与早孕孕周相关。

关键词: 杀伤细胞免疫球蛋白样受体；自然流产；CD158；蜕膜；表达

Abstract:

Objective To investigate the expression of CD158 receptors in deciduas of women with early recurrent spontaneous abortion (RSA). Methods The decidual tissue samples were taken by uterine curetting from 30 RSA patients and 30 healthy controls, and divided into three groups by pregnancy days. Mononuclear cells were isolated from deciduas by ficoll density gradient centrifugation. Expressions of the receptors CD56+, CD16-, CD158a and CD158b were detected by three-colour flow-cytometric analysis. Two-tailed Fisher’s exact test and Mann-Whitney U tests were used to compare variables among groups. Spearman’s correlation and Kruskal-Wallis tests were used for association. Results ① CD56+ cells were significantly decreased in women with RSA when compared with the normal controls (P=0.007), and CD56+CD16- cells were significantly decreased in women with RSA when compared with the normal controls(P＜0.05).② CD158a expressed by CD56+CD16- cells were significantly decreased in women with RSA compared with the control subjects(P=0.026). ③ CD158a expressed by CD56+CD16- cells were significantly decreased in women with RSA especially in 7~10 gestational weeks(P=0.010). ④ The expressions of CD158a and CD158b in normal deciduas were related to CD56(P＜0.01). Conclusions Decreased CD56+CD16- expressions are found in mononuclear cells of deciduas of women with RSA. CD158a expressed by CD56+CD16- cells may explain the pathogenesis of RSA and may be related to gestational age.

Key words: Killer cell immunoglobulinlike receptor(KIR); Abortion, spontaneous； CD158； Deciduas；Expression

中图分类号:

R714.21

王珊1，陈子江2，许成岩2，宋辉3，赵跃然4，傅艺冰1 . 不明原因早期复发性自然流产患者蜕膜组织CD158受体的表达[J]. 山东大学学报(医学版), 2012, 50(8): 103-107.

WANG Shan1， CHEN Zi-jiang2， XU Cheng-yan2， SONG Hui3， ZHAO Yue-ran4， FU Yi-bing1. Expression of CD158 in deciduas of women with early unexplained
recurrent spontaneous abortion[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(8): 103-107.

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