EV71临床分离株BALB/c小鼠致病特点分析

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (8): 35-.

EV71临床分离株BALB/c小鼠致病特点分析

宋楠楠1，张颖2，李鹏1，岳盈盈1，李志会1，盖中涛2，孟红1

1.山东省医学科学院基础医学研究所山东省罕少见病重点实验室，济南 250062；
2.济南市传染病医院6科，济南 250021

收稿日期:2011-09-09 出版日期:2012-08-10 发布日期:2012-08-10
通讯作者: 孟红(1960- )，女，研究员，主要从事医学病毒学研究。 E-mail：menghyky@yahoo.com.cn
作者简介:宋楠楠(1980- )，女，硕士研究生，助理研究员，主要从事病原生物学研究
基金资助:
国家自然科学基金(81000720)；山东省自然科学基金(ZR2011HQ030)

Analysis of the pathogenic characteristics of BALB/c
mice infected with enterovirus 71 isolates

ONG Nan-nan1, ZHANG Ying2, LI Peng1, YUE Ying-ying1, LI Zhi-hui1,
GAI Zhong-tao2, MENG Hong1

1. Institute of Basic Medicine， Shandong Academy of Medical Sciences; Key Laboratory of
Rare and Uncommon Disease, Jinan 250062, China；
2. The Sixth Department, Jinan Infectious Diseases Hospital, Jinan 250021, China

Received:2011-09-09 Online:2012-08-10 Published:2012-08-10

摘要/Abstract

摘要：

目的将肠道病毒71型(EV71)临床分离株接种小鼠观察其致病特点。方法将6株EV71临床株分别腹腔接种1日龄BALB/c小鼠，观察小鼠表现以及脑、肺脏、骨骼肌组织病理变化；采用免疫组化和RT-PCR方法，检测组织中病毒抗原和基因片段。结果临床分离的6株EV71(C4亚型)接种1日龄小鼠均出现病症，其中JN200804株实验小鼠症状较重，小鼠脑组织和骨骼肌可观察到细胞空泡变性、淋巴细胞浸润等病理变化；JN200804株感染组小鼠脑内检测到EV71抗原和基因片段。结论 6株EV71临床株均可感染小鼠并致病，重者出现松弛型麻痹并死亡，轻者出现震颤、麻痹但可恢复至正常。

关键词: 肠道病毒C型，人；免疫组织化学；逆转录聚合酶链反应；基因；小鼠，近交BALB C

Abstract:

Objective To study the characteristics of enterovirus 71(EV71)isolates in vivo, and to provide information for the research of EV71. Methods One-day old BALB/c mice were infected with 6 EV71 strains isolates, and symptoms were observed. All mice were sacrificed at 7d after the infection to collect organs and tissues for the pathological examination, RT-PCR detection and immunohistochemistry. Results 6 viruses were isolated from stool samples, and identified as C4 genotype of EV71. All of them could induce lesions of one-day old mice. The JN200804 strain induced flaccid paralysis on mice 5 days after the infection. Histopathologically, lymphocytic infiltration and some other pathologic changes were observed in the infected mice brain, lung and skeletal muscles. mRNA and antigens was detected in the brain of mice infected with JN200804. Conclusion 6 strain of EV71 isolates can cause the disease on BALB/c mice, including the paralysis, muscular tremor, and death of infected mice.

Key words: Enterovirus C, human; Immunohistochemistry; Reverse transcriptase polymerase chain reaction; Genes; Mice, inbred BALB C

中图分类号:

R373.2

宋楠楠1，张颖2，李鹏1，岳盈盈1，李志会1，盖中涛2，孟红1. EV71临床分离株BALB/c小鼠致病特点分析[J]. 山东大学学报(医学版), 2012, 50(8): 35-.

ONG Nan-nan1, ZHANG Ying2, LI Peng1, YUE Ying-ying1, LI Zhi-hui1, GAI Zhong-tao2, MENG Hong1. Analysis of the pathogenic characteristics of BALB/c
mice infected with enterovirus 71 isolates[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(8): 35-.

参考文献

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