亚低温对过氧化氢损伤后脑微血管内皮细胞NF-κB表达的影响

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (8): 10-.

亚低温对过氧化氢损伤后脑微血管内皮细胞NF-κB表达的影响

李彦芬，王敏忠，张镛，杜怡峰，郭守刚，李春霞，刘雪平

山东大学附属省立医院神经内科，济南 250021

收稿日期:2011-11-22 出版日期:2012-08-10 发布日期:2012-08-10
通讯作者: 王敏忠(1966- )，男，教授，硕士生导师，主要从事脑血管病和痴呆的研究。 E-mail：wmzwlq@163.com
作者简介:李彦芬(1984- )，女，硕士研究生，主要从事脑血管病研究。
基金资助:
山东省卫生厅计划基金(2007HW093)；山东省科技攻关计划基金(2010G0020216)

Effects of mild hypothermia on expression of nuclear transcription
factor-kappa B (NF-κB) in rat cerebral microvascular
endothelial cells injured by hydrogen peroxide

LI Yan-fen, WANG Min-zhong, ZHANG Yong, DU Yi-feng, GUO Shou-gang,
LI Chun-xia, LIU Xue-ping

Department of Neurology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2011-11-22 Online:2012-08-10 Published:2012-08-10

摘要/Abstract

摘要：

目的研究亚低温对过氧化氢损伤后脑微血管内皮细胞核转录因子(NF-κB)表达的影响。方法分离并培养大鼠脑微血管内皮细胞，用过氧化氢损伤的方法建立脑出血后血肿周围炎症反应内皮细胞模型。将细胞分为实验组(ICH)和对照组(C)。ICH组分为过氧化氢损伤常温组(ICH+N)和过氧化氢损伤亚低温组(ICH+H)，C组分为常温对照组(C+N)和亚低温对照组(C+H)。采用Western blot技术检测各组NF-κB的表达水平。结果实验组NF-κB的表达水平均高于各自对照组(P<0.01)， ICH+N组蛋白表达水平高于ICH+H组(P<0.01)。结论亚低温可有效降低过氧化氢损伤的脑微血管内皮细胞NF-κB的表达，从而减轻血肿周围炎症反应,发挥其脑保护作用。

关键词: 低温；脑出血；NFκB；内皮，血管；炎症

Abstract:

Objective To investigate the influence of mild hypothermia on the expression of nuclear factor-kappa B(NF-κB) in cerebral microvascular endothelial cells injured by hydrogen peroxide. Methods The cells of rat cerebral microvascular endothelial were separated and cultured, and the cell model of inflammatory response after cerebral hemorrhage was established by the method of hydrogen peroxide injury. Cells were divided into the experiment group(ICH) and the control group(C). Each group was divided into the normothermia group(N ) and the mild hypothermia group(H). The expression of NF-κB was detected by western blotting after hydrogen peroxide intervention. Results The expression of NF-κB obviously increased in the experiment group compared with the control group(P<0.01). While the level of NF-κB was higher in the normothermia group than that in the mild hypothermia group(P<0.01). Conclusion Mild hypothermia may protect perihematoma tissues through relieving the inflammation by inhibiting NF-κB over-expression.

Key words: Hypothermia; Cerebral hemorrhage; NF-kappa B; Endothelium, vascular; Inflammation

中图分类号:

Q952.6

李彦芬，王敏忠，张镛，杜怡峰，郭守刚，李春霞，刘雪平. 亚低温对过氧化氢损伤后脑微血管内皮细胞NF-κB表达的影响[J]. 山东大学学报(医学版), 2012, 50(8): 10-.

LI Yan-fen, WANG Min-zhong, ZHANG Yong, DU Yi-feng, GUO Shou-gang, LI Chun-xia, LIU Xue-ping. Effects of mild hypothermia on expression of nuclear transcription
factor-kappa B (NF-κB) in rat cerebral microvascular
endothelial cells injured by hydrogen peroxide[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(8): 10-.

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