三种镇静催眠药对阿司匹林酯酶活性的影响

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (4): 151-.

三种镇静催眠药对阿司匹林酯酶活性的影响

周慧1,于宗琴2,李军3,李刚1,纪洪生1

山东大学 1. 附属省立医院麻醉科，济南 250021； 2. 药学院，济南 250012；
3. 附属省立医院临床药理中心，济南 250021

收稿日期:2009-11-23 出版日期:2010-04-16 发布日期:2010-04-16
通讯作者: 李刚（1951- ），男，主任医师，主要从事麻醉与外科重症监护的研究。 Email:wdkyxx@yahoo.cn
作者简介:周慧（1984- ），女，硕士研究生，主要从事麻醉与外科重症监护的研

Effects of three kinds of sedative hypnotics on activity of aspirin esterase

ZHOU Hui1, YU Zongqin2, LI Jun3, LI Gang1, JI Hongsheng1

1. Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021， China；
2. Pharmacy College of Shandong University， Jinan 250012， China；
3． The Center of Clinical Pharmacology， Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021， China

Received:2009-11-23 Online:2010-04-16 Published:2010-04-16

摘要/Abstract

摘要：

目的通过体外培养体法考察镇静催眠药（安定、咪达唑仑和苯巴比妥）对阿司匹林酯酶活性的影响。方法采用实验室建立的阿司匹林酯酶体外代谢系统，用高效液相色谱直接进样法测定培养体系中阿司匹林（ASA）和水杨酸(SA)的浓度，以SA/(ASA+SA)比值反应阿司匹林酯酶的活性。通过在培养体系中加入或不加安定、咪达唑仑和苯巴比妥时阿司匹林酯酶活性的变化，分析上述镇静催眠药对阿司匹林酯酶活性的影响。结果安定、咪达唑仑、苯巴比妥对阿司匹林酯酶活性的影响分别为-10.71%（P＞0.05）、-27.94%（P＜0.05）和8.83%(P＜0.05）,咪达唑仑对阿司匹林酯酶的活性有一定的抑制作用，苯巴比妥对阿司匹林酯酶的活性有一定的诱导作用。结论咪达唑仑、苯巴比妥与阿司匹林合用时应注意药物间的相互作用。

关键词: 阿司匹林酯酶；色谱法，高效液相；镇静催眠药；阿司匹林

Abstract:

Objective To study the effects of sedative hypnotics on the activity of aspirin esterase in vitro. Methods The metabolic system of aspirin esterase in vitro was established in the laboratory. The separated plasma specimens were treated with the sedative hypnotics, and then the concentrations of aspirin and its metabolite (salicylic acid）in the plasma were determined by RP- HPLC. The ratio of salicylic acid (SA) and aspirin (ASA) represented the activity of aspirin esterase. Results The effects of diazepam, midazolam and phenobarbital on the activity of aspirin esterase were -10.71%（P＞0.05）, -27.94%（P＜0.05）and 8.83%(P＜0.05） respectively. The activity of aspirin esterase was inhibited by midazolam, while it was induced by phenobarbital. Conclusion The interaction between sedative hypnotics and aspirin should be paid careful attention to in clinical application.

Key words: Chromatography, High performance liquid; Sedative hypnotics drugs; Aspirin

中图分类号:

R917

周慧1,于宗琴2,李军3,李刚1,纪洪生1. 三种镇静催眠药对阿司匹林酯酶活性的影响[J]. 山东大学学报(医学版), 2010, 48(4): 151-.

ZHOU Hui1, YU Zongqin2, LI Jun3, LI Gang1, JI Hongsheng1. Effects of three kinds of sedative hypnotics on activity of aspirin esterase[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(4): 151-.

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