您的位置:山东大学 -> 科技期刊社 -> 《山东大学学报(医学版)》

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (4): 5-9.

• 论文 • 上一篇    下一篇

胰岛素抵抗大鼠血管AGEs水平及其损伤机制和吡格列酮的保护作用

侯亮,刘雪平,袁树华,王美霞,徐松   

  1. 山东大学附属省立医院保健神经科,   济南 250021
  • 收稿日期:2009-10-08 出版日期:2010-04-16 发布日期:2010-04-16
  • 通讯作者: 刘雪平(1962- ),女,博士,主任医师,主要从事脑血管疾病及认知障碍的研究。 Email:Lxp6133@yahoo.com.cn
  • 作者简介:侯亮(1982- ),男,硕士研究生,主要从事老年神经病学的研究。
  • 基金资助:

    山东省科学技术发展计划项目(2006GGB14630)

Level and injury mechanism of AGEs in the vascular system of insulin resistance rats and protective effects of Pioglitazone

HOU Liang, LIU Xueping, YUAN Shuhua, WANG Meixia, XU Song   

  1. Department of Senile Neurology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China
  • Received:2009-10-08 Online:2010-04-16 Published:2010-04-16

摘要:

目的  探讨胰岛素抵抗大鼠血管AGEs水平及其损伤机制和吡格列酮的保护作用。方法  选6~8 周龄Wistar大鼠随机分为对照组(NC组,n=10)、胰岛素抵抗组(IR组,n=13)和吡格列酮组(PIO组,n=13)。后两组建立胰岛素抵抗模型,模型成功后PIO组给予吡格列酮[10mg/(kg·d)]干预,12周后采用免疫荧光技术检测血管壁AGEs表达;应用RT-PCR法检测RAGE 、NADPH氧化酶 p47phox mRNA 的表达;免疫组织化学技术检测RAGE、磷酸化NF-Кb蛋白的表达。结果  与IR组比较,PIO组AGEs的表达减弱,而两组明显高于NC组;IR组和PIO组RAGE、NADPH氧化酶P47phox mRNA表达较NC组明显升高(P< 0.01),而PIO组上述指标表达较IR组减弱(P<0.05);PIO组RAGE、磷酸化NF-Кb蛋白表达较IR组减弱(P<0.05),但两组均明显强于NC组(P<0.01)。结论  胰岛素抵抗大鼠血管AGEs及RAGE表达增多,继而促进氧化应激及炎症反应导致血管损伤;吡格列酮能通过减少AGEs、RAGE生成而抑制氧化应激及炎症反应,改善血管损伤。

关键词: 胰岛素抵抗;糖基化终末产物;吡格列酮;氧化应激

Abstract:

Objective  To explore the level and injury mechanism of advanced glycosylation end products(AGES) in the vascular system of rats with insulin resistance and the protective effects of Pioglitazone. Methods  6-8-week-old Wistar rats were randomly divided into the control group (NC group,  n=10), the insulin resistance group (IR group,  n=13) and the Pioglitazone group (PIO group,  n=13).The latter two groups were developed into the insulin resistance model. The PIO group were given Pioglitazone 10mg/ (kg·d) by gavage for 12 weeks.  Expressions of advanced glycosylation end products were detected by immunofluorescence. The mRNA expression levels of RAGE and NADPH oxidase p47phox were detected by RT-PCR respectively. Immunohistochemistry was performed to detect expression of RAGE and phosphor-NF-Кb protein. Results  Compared with the IR group,  expression of AGEs decreased in PIO group. Expression of AGEs in the two groups was significantly higher than in the NC group. RAGE, NADPH oxidase P47phox, and mRNA expressions in the IR group and the PIO group were significantly higher than that in the NC group(P<0.01).But, in the PIO group  expression of these indicators  decreased in comparison with the IR group (P<0.05).RAGE and phosphor-NF-Кb protein expression in the PIO group were reduced in comparison with the IR group (P<0.05), while in the other two groups, RAGE and phosphor-NF-Кb protein expressions  were significantly stronger than the NC group (P<0.01). Conclusions  Expression of AGEs protein is increased in the vascular system of rats with insulin resistance, which can promote oxidative stress and inflammatory response. Pioglitazone can inhibit  oxidative stress and the inflammatory response by decreasing  expression of AGEs and RAGE and the activation of NF-Кb to reverse the vascular injury.

Key words: Insulin resistance; Advanced glycosylation end products; Pioglitazone; Oxidativestress

中图分类号: 

  • R587.2
[1] 毕大鹏1,嵇高德1,张洪星2 . 通心络胶囊对早期糖尿病肾病患者胱抑素C的影响[J]. 山东大学学报(医学版), 2013, 51(7): 67-69.
[2] 周雁1,郭立新1,周璐2,汪耀1,牟忠卿1,王晓霞1,于冬妮1,张丽娜11,周璐2,汪耀1,牟忠卿1,王晓霞1,于冬妮1,张丽娜1. 老年2型糖尿病患者周围神经病变的影响因素[J]. 山东大学学报(医学版), 2012, 50(6): 1-4.
[3] 王倩1,张莹1,侯为开1,张莉2,徐华3,王鹏4,于珊1, 刘骞1,黄庆先1. 2型糖尿病性腹泻与肠神经系统病变关系的探讨[J]. 山东大学学报(医学版), 2011, 49(8): 13-.
[4] 张月华,赵新国,岳玉国,吕英俊. 诺和锐30特充强化治疗初诊2型糖尿病疗效观察[J]. 山东大学学报(医学版), 2010, 48(4): 160-.
[5] 李文斌1,崔美玉1,许冬梅1,关广聚2. 二基肽酶IV酶活性与糖尿病肾病的相关性[J]. 山东大学学报(医学版), 2010, 48(3): 12-14.
[6] 李倩,汪翼,孙书珍,陈瑶,王立俊. 糖尿病大鼠心肌胶原代谢的变化及与MMP-2/TIMP-2表达的关系[J]. 山东大学学报(医学版), 2010, 48(3): 1-6.
[7] 李宪花 高海青 李保应 程梅 许玲 胡昭. 葡萄子原花青素对糖尿病肾病大鼠非酶糖基化终产物和骨形态蛋白7表达的影响[J]. 山东大学学报(医学版), 2009, 47(5): 16-22.
[8] 孙洁 任建民 陈超 刘昌盛 司庆盈. 糖尿病合并冠心病与白蛋白尿的相关性研究[J]. 山东大学学报(医学版), 2009, 47(5): 102-105.
[9] . 下肢动脉病变评分与糖尿病足1年截肢率的相关性研究[J]. 山东大学学报(医学版), 2009, 47(8): 10-13.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!