丙酮酸乙酯对脓毒症大鼠小肠黏膜屏障的保护作用及其机制

doi:10.6040/j.issn.1671-7554.0.2013.680

山东大学学报（医学版）

丙酮酸乙酯对脓毒症大鼠小肠黏膜屏障的保护作用及其机制

张庆伟1，王春亭2，王启志2，张琳2，王鹏2，孟玫2

1.山东医学高等专科学校，山东济南 250002； 2.山东大学附属省立医院重症医学科，山东济南 250021

收稿日期:2013-12-13 出版日期:2014-05-10 发布日期:2014-05-10
通讯作者: 孟玫 E-mail：15168887139@163.com

Protective effect and mechanism of ethyl pyruvate on the small intestine barrier in rats with sepsis

ZHANG Qingwei1, WANG Chunting2, Wang Qizhi2, ZHANG Lin2, WANG Peng2, MENG Mei2

1. Shandong Medical College, Jinan 250002， Shandong， China; 2. Intensive Care Unit,
Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong， China

Received:2013-12-13 Online:2014-05-10 Published:2014-05-10

摘要/Abstract

摘要： 目的探讨丙酮酸乙酯(EP)对脓毒症大鼠小肠黏膜屏障功能的影响及其机制。方法采用盲肠结扎穿孔术(CLP)将85只Wistar大鼠制备脓毒症模型，并随机分成脓毒症林格氏液组(Rin组)、脓毒症EP治疗组(EP组)、假手术对照组。观察各组大鼠小肠黏膜组织变化和细菌移位情况，检测小肠黏膜微循环血流量和髓过氧化物酶的活性变化，以及诱生型一氧化氮合酶(iNOS)mRNA表达水平和肠腔内的肥大细胞蛋白酶(RMCPⅡ)量的变化，统计各组大鼠的96h生存率。结果 Rin组小肠黏膜损伤较重，EP组小肠黏膜较Rin组有改善，细菌移位集落数减少；假手术对照组未发现细菌移位；与假手术对照组比较，Rin组小肠黏膜的血流减少(P<0.001)，MPO表达增高(P<0.001)，RMCPⅡ浓度增高显著(P<0.001)，iNOS/β-actin比值升高(P<0.001)；与Rin组比较，EP组腹腔注射EP后能改善小肠黏膜微循环血流量(P<0.001)，抑制MPO(P<0.05)和iNOS高表达(P<0.05)，对RMCPⅡ影响不大(P>0.05)，可提高脓毒症大鼠的96h生存率(P<0.01)。结论 EP可能是通过减少肠道组织MPO、iNOS表达，降低脓毒症大鼠肠道黏膜细菌移位，显著改善微循环血流，对脓毒症大鼠的小肠黏膜屏障有一定的保护作用。

关键词: 脓毒症, 小肠黏膜屏障, 丙酮酸乙酯, Wistar, 大鼠

Abstract: Objective To explore the effects of ethyl pyruvate (EP) on the small intestine barrier in rats with sepsis and its possible mechanism. Methods A total of 85 Wistar rats with sepsis induced by cecal ligation and puncture surgery (CLP) were randomly divided into 3 groups: Ringers administration group (Rin group), the septic group with ethyl pyruvate administration (EP group) and sham-operated group(the control group). The histological changes and bacterial translocation in the ileal architecture microvascular were observed, perfusion of small intestine, activity of myeloperoxidase (MPO), the level of inducible nitric oxide synthase (iNOS) mRNA and rat mast cell protease-Ⅱ (RMCP Ⅱ) were tested, and the survival rate of rats was calculated. Results The Rin group had more severe injury of small intestinal mucosa, while the EP group had better morphological changes, and fewer bacterial colonies shift compared with the Rin group. Compared with the control group, the Rin group had weaker blood flow, higher expression of MPO, RMCP Ⅱ, and iNOS (all P<0.001). Compared with the Rin group, EP group had enlarged blood flow, decreased expressions of MPO and iNOS, and higher survival rate of animals (all P<0.001). Conclusion EP can ameliorate the morality rate of sepsis and improve the microcirculation by decreasing MPO and iNOS expressions in the small intestine, which may protect the small intestine barrier of septic rats.

Key words: Ethyl pyruvate, Wistar, Rats, Small intestine barrier, Sepsis

中图分类号:

R631+.4

张庆伟1，王春亭2，王启志2，张琳2，王鹏2，孟玫2. 丙酮酸乙酯对脓毒症大鼠小肠黏膜屏障的保护作用及其机制[J]. 山东大学学报（医学版）, 2014, 52(5): 30-34.

ZHANG Qingwei1, WANG Chunting2, Wang Qizhi2, ZHANG Lin2, WANG Peng2, MENG Mei2. Protective effect and mechanism of ethyl pyruvate on the small intestine barrier in rats with sepsis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 30-34.

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丙酮酸乙酯对脓毒症大鼠小肠黏膜屏障的保护作用及其机制

Protective effect and mechanism of ethyl pyruvate on the small intestine barrier in rats with sepsis

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