化淤理肺方对肺纤维化大鼠MMP2和TIMP1表达的影响

山东大学学报(医学版)

化淤理肺方对肺纤维化大鼠MMP2和
TIMP1表达的影响

王琴¹，刘世青²，李长生³，鹿伟⁴

(1. 山东大学医学院，济南 250012； 2. 山东大学附属千佛山医院呼吸科，济南 250014； 3. 山东大学附属千佛山医院中医科，济南 250014； 4. 山东大学附属千佛山医院病理科，济南 250014)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-16 发布日期:2009-02-16
通讯作者: 刘世青

Effects of Huayulifei on the expressions of MMP2 and TIMP1 in
rats with bleomycininduced pulmonary fibrosis

WANG Qin¹, LIU Shiqing², LI Changsheng³, LU Wei⁴

(1. School of Medicine, Shandong University, Jinan 250012, China; 2. Department of Respiratory;
3. Department of Traditional Chinese Medicine; 4. Department of Pathology,
Qianfoshan Hospital, Shandong University, Jinan 250014, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-16 Published:2009-02-16
Contact: LIU Shiqing

摘要/Abstract

摘要： 目的探讨化淤理肺方对肺纤维化的干预作用和对MMP2/TIMP1表达的影响。方法肺纤维化Wistar雄性大鼠84只,随机分成4组:中药组、激素组、模型组和对照组。所有大鼠第7、14、28天各处死7只。经HE染色评价肺组织肺泡炎和肺纤维化程度，免疫组化检测法检测MMP2与TIMP1的表达。结果① 肺泡炎、肺纤维化程度评价：模型组肺泡炎和肺纤维化程度较重,且胶原纤维沉积较多;中药组可见炎性细胞较少,肺泡结构基本正常,肺泡炎与肺纤维化较轻;激素组肺泡炎及纤维化与中药组相似。 ② MMP2蛋白的表达：中药组第7、14、28天较模型组表达明显减轻，于第14天达到高峰，此后降低，表达水平与激素组相似。③ TIMP1蛋白表达：中药组在这3个时段的表达均较对照组增高, 但均较模型组低，且呈逐渐升高趋势。结论化淤理肺方能减轻博来霉素诱导的大鼠肺纤维化,可能是通过调节MMP2、TIMP1途径起作用。

关键词: 肺纤维化, 化瘀理肺方, 基质金属蛋白酶2, 基质金属蛋白酶组织抑制物, 大鼠,Wistar

Abstract: To evaluate the effects and mechanism of Huayulifei on blemycininduced pulmonary interstitial fibrosis in rats. MethodsRats were randomly divided into the control group, the model group, the Huayulifei group and the hydrocortisone group. Rat pulmonary fibrosis was induced by blemycin(5?mg/kg) in the experimental groups and 0.9% saline in the control group. Rats were killed on the 7th，14th and 28th days. Expressions of MMP2 and TIMP1 proteins were determined by an immunohistochemical technique. Results① The airsacculitis and fibrosis of lungs in the model group were fairly severe which had much deposition of collagen fibers. In the Huayulifei group which had few inflammatory cells, the alveoli structure was nearly normal, and most of the airsacculitis and fibrosis of lungs were mild. In the Hydrocortisone group, alleviation of airsacculitis and fibrosis of lungs were similar with those of the Huayulifei group. ② In the Huayulifei group, expressions of MMP2 protein and TIMP1 protein were significantly lighter than those in the model group. ConclusionHuayulifei can ameliorate blemycininduced pulmonary fibrosis in rats, and it may partly work by affecting the expression of MMP2/TIMP1.

Key words: Pulmonary fibrosis, Huayulifei, Matrix metallopeptidase2, Tissue inhibitor of metalloproteinase1, Rats,Wistar

中图分类号:

Ｒ563

王琴1 ，刘世青2 ，李长生3 ，鹿伟4
. 化淤理肺方对肺纤维化大鼠MMP2和
TIMP1表达的影响[J]. 山东大学学报(医学版), 2009, 47(02): 14-17.

WANG Qin¹, LIU Shiqing², LI Changsheng³, LU Wei⁴
. Effects of Huayulifei on the expressions of MMP2 and TIMP1 in
rats with bleomycininduced pulmonary fibrosis
[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(02): 14-17.

参考文献

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