一氧化氮在肥胖大鼠中的变化及其与肾脏损害的关系

山东大学学报(医学版)

一氧化氮在肥胖大鼠中的变化
及其与肾脏损害的关系

梁珺¹，孙书珍²，李倩²，陈瑶²，汪翼²

(1. 聊城市人民医院儿科, 山东聊城 252000； 2. 山东大学附属省立医院儿科, 济南 250021）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-01-16 发布日期:2009-01-16
通讯作者: 孙书珍

Changes of nitric oxide and relationship between nitric oxide and renal injury in obese rats

LIANG Jun¹, SUN Shuzhen², LI Qian², CHEN Yao²,　WANG Yi²

(1. Department of Pediatrics, Liaocheng People′s Hospital, Liaocheng 252000, Shandong, China; 2. Department of Pediatric Nephrology, Shandong Provincial Hospital Affiliated to Shandong University， Jinan 250021，China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-01-16 Published:2009-01-16
Contact: SUN Shuzhen

摘要/Abstract

摘要： 目的探讨一氧化氮（NO）在肥胖大鼠中的变化及其与肾脏损伤的关系。方法将16只健康雄性Wistar 大鼠随机分成对照组（n=8）及肥胖组（n=8），分别给予普通饮食及高脂饮食。喂养20周后处死全部大鼠，观察两组大鼠体重指数（Lee指数）、左肾指数、血总胆固醇（TCH）、甘油三酯(TG)、低密度脂蛋白胆固醇(LDLC)、高密度脂蛋白胆固醇(HDLC)、血糖(Glu)、24?h尿蛋白定量(UPE)及内生肌酐清除率（Ccr）的变化；测定两组大鼠血、肾组织匀浆NO含量以及尿NO代谢产物排泄量（UNOxV）；同时观察两组大鼠肾组织形态学改变，并计算肾小球平均截面积。结果肥胖组大鼠Lee指数、左肾指数、血TCH、TG、LDLC、UPE及Ccr均高于对照组（P<0.01）；HDLC、血、肾组织匀浆NO水平及UNOxV均低于对照组（P<0.01）。肥胖组大鼠肾小球存在系膜细胞增生，球囊粘连，间质小动脉内膜增生、部分透明变性，肾小球平均截面积明显增大（P<0.01）。血、肾组织NO水平与TG、TCH、LDLC、UPE、Ccr、肾小球平均截面积呈负相关（r分别为-0.869?4,-0.788?1;-0.795?4,-0.783?5;-0.918?2,-0.873?0;-0.894?0,-0.903?5;-0.612?2,-0.553?5;-0.641?1,-0.599?6），而与HDLC及UNOxV呈正相关（r分别为0.934?9,0.930?7;0.855?7,0.822?1）。结论肥胖大鼠存在明显的血管、微血管内皮功能障碍以及肾脏损害，前者与脂代谢紊乱有关，后者可能与肥胖大鼠体内NO降低有关。UNOxV的测定作为一种无创伤性的检测指标可间接了解肥胖大鼠体内NO的水平。

关键词: 肥胖, 一氧化氮, 肾损害, 大鼠,Wistar

Abstract:

To explore the changes of nitric oxide and its role in renal injury in obese rats. MethodsSixteen healthy male Wistar rats were randomly divided into the control(n=8) and obese group(n=8) which were respectively given an ordinary diet and a high fat diet. After 20 weeks, all rats were killed. The Lee index, left renal index, the content of cholesterol total(TCH), triglyceride(TG), low density lipoprotein cholesterol(LDLC) , high density lipoprotein cholesterol(HDLC) and urine protein quantitation in 24 hours(UPE) and creatinine clearance(Ccr) were detected. Nitric oxide (NO) content in blood , renal tissue and urinary nitrite+nitrate excretion (UNOxV) were acquired by a nitrate disoxidation method. Histomorphology of kidneys was examined by an optical microscope, and the glomerulum section area was determined by image analysator. ResultsIn the obese group, the index of Lee, left renal index, cholesterol total(TCH), triglyceride (TG), low density lipoprotein cholesterol(LDLC), urine protein quantitation in 24 hours and creatinine clearance(Ccr) were significantly higher (P<0.01), while high density lipoprotein cholesterol(HDLC), nitric oxide(NO) content in blood, renal tissue and urinary nitrite+nitrate excretion (UNOxV) were lower(P<0.01). In the obese group, mesangial cell proliferation, Bowman′s space proprius adhesion, interstitial arteriole endothelial hyperplasia, hyalinosis and glomerular average section area were obviously increased (P<0.01). Relative analysis demonstrated that the NO contents of the serum and renal tissue were negatively correlated with TG, TCH, LDLC, UPE, Ccr and glomerulum section area（r=-0.869?4,-0.788?1; -0.795?4,-0.783?5; -0.918?2,-0.873?0; -0.894?0, -0.903?5; -0.612?2, -0.553?5; -0.641?1, -0.599?6）,　while positively correlated with HDLC and UNOxV（r=0.934?9,0.930?7; 0.855?7,0.822?1）. ConclusionThere were evident functional disturbances of blood vessel endothelium and capillary vessel endothelium and renal damage in the obese group. The former is relevant to the conspicuous lipid metabolism disorder, and the latter is relevant to insufficient NO synthesis. As a noninvasive detection indicator, UNOxV can indirectly reflect NO synthesis in obese rats.

Key words: Obesity, Nitric oxide, Renal injury, Rats,Wistar

中图分类号:

R589.2

梁珺1 ，孙书珍2 ，李倩2 ，陈瑶2 ，汪翼2
. 一氧化氮在肥胖大鼠中的变化
及其与肾脏损害的关系[J]. 山东大学学报(医学版), 2009, 47(01): 42-44.

LIANG Jun¹, SUN Shuzhen², LI Qian², CHEN Yao²,　WANG Yi²
. Changes of nitric oxide and relationship between nitric oxide and renal injury in obese rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(01): 42-44.

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