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山东大学学报(医学版) ›› 2209, Vol. 47 ›› Issue (6): 25-27.

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颞下颌关节骨关节病动物模型的建立

赵立星 宋代辉 魏魁杰 殷恺   

  1. 1.天津市第一中心医院, 天津 300192; 2. 山东大学口腔医学院,  济南 250012;3. 湖州师范学院医学院, 浙江 湖州  313000
  • 收稿日期:2007-04-23 出版日期:2009-06-16 发布日期:2209-06-25
  • 通讯作者: 宋代辉,教授. E-mail:sdh@sdu.edu.cn
  • 作者简介:赵立星(1972- ),男,硕士,主治医师,主要从事口腔颌面外科的基础与临床研究。E-mail:zlx7222@tom.com

An animal model of osteoarthritis on the temporomandibular joint

 ZHAO Li-Xing, SONG Dai-Hui, WEI Kui-Jie, YIN Kai   

  1. 1. Tianjin First Central Hospital, Tianjin 300192, China; 2. School of Stomatology, Shandong University, Jinan 250012,  China; 3. Faculty of Medicine,Huzhou Teachers College, Huzhou 313000, Zhejiang, China
  • Received:2007-04-23 Online:2009-06-16 Published:2209-06-25

摘要:

目的 建立全颞下颌关节骨关节病(TMJOA)实验动物模型。  方法 选择8只新西兰大白兔,在大白兔的右侧颞下颌关节上腔以及下腔注射注射2%木瓜蛋白酶各0.2?mL,注射两次,3天一次,左侧为对照,注射后第1、3、5周取材,对TMJ进行组织病理学观察。  结果 注射木瓜蛋白酶早期,颞骨关节面、关节盘、髁状突软骨即出现明显的骨关节病的改变;后期出现关节软骨纤维层剥脱,关节软骨以及关节盘中的裂隙明显,局部颞骨关节面和髁状突出现关节软骨剥脱等损害 结论  实验结果表明,颞下颌关节上、下腔注入木瓜蛋白酶是造成全颞下颌关节退行性改变动物模型的有效方法,该方法快速、简单、重复性好。

关键词: 颞下颌关节骨关节病;病理学;模型,动物

Abstract:

Objective To develop an animal model of the total temporomandibular joint (TMJ) osteoarthritis in rabbits.  Methods Eight New Zealand white rabbits were selected. The upper and lower compartments of the right TMJ of the white rabbits was injected with 0.2mL 2% papain. Three days later, the same amount of papain was injected at the same place, and the left TMJ was used as a control. The animals were killed on the 1st, 3rd and 5th week. The TMJ and the nearby tissues were cut off and then the TMJ was pathologically examined.  Results Under light microscopy, in the early stage serious osteoarthritis that had happened on the rightTMJ was found one week after treatment and the pathological changes were significant in the temporal surface, disc and condyle. The cartilage was potholed and the stratum fibrosum was denuded, and the collagen fiber was loosened and fragmented in the later stage.  Conclusion   Papain injected into the upper and lower compartments of the TMJ is efficient to make a retrograde model of TMJ, and this method is simple and repeatable.

Key words: Osteoarthritis of the temporomandibular joint; Ppathology; Model, animal

中图分类号: 

  • R782
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[3] 赵立星 宋代辉 魏魁杰 殷恺. 颞下颌关节骨关节病动物模型的建立[J]. 山东大学学报(医学版), 2009, 47(6): 25-27.
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