Abstract: Objective To investigate the effects of Na₂SeO₃ combined with Vit C on the activities of SOD, GSH-PX， the content of MDA in the liver and kidney of the mice, and mitotic division of vaginal epitheliums. Methods Female Kunming mice were used to establish the mitotic division model of vaginal epitheliums. They were randomly divided into eight groups: the negative control, the positive control, the low, middle and high dosage of Na₂SeO₃, and the low, middle and high dosage of Na₂SeO₃ and Vit C. The corresponding reagent was administered for twelve consecutive days.　 One hour after last administration, colchicines was injected into the abdomen of the mice, and then the mice were sacrificed by taking the cervical vertebrae off. Kidney and liver tissues were made into homogenate for determining the enzymatic activity. Simultaneously, the vaginal mitotic division was observed under a light microscope. ResultsActivities of SOD and GSH-PX in livers and activities of GSH-PX in kidneys in each selenium replenished group and methotrexate group were significantly higher than in the negative control group(P＜0.05), while the MDA content difference was not statistically significant among them. Both Na₂SeO₃ and Na₂SeO₃-Vit C could obviously inhibit the mitoses of vaginal epithelial cells(P＜0.05), but no statistically significance was found between them and the positive control group. Conclusion Na₂SeO₃ could raise activities of GSHPX in liver and kidney, promote the effect of antioxidizing system and thus effectively inhibits the mitotic division of vaginal epitheliums. Na₂SeO₃ and Vit C may not act in coordination.

Key words: Selenium, Superoxide dismutase, Psoriasis, Mice