JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Co-effects of human cytomegalovirus infection and advanced glycation end products on vascular endothelial cells

LI Shu-ying1， WU Jian-min1，ZHOU Ya-bin1，ZHANG Feng-lei1，CHENG Yi-zhe1，YU Han1，QI Mei1，TANG Wei1，王红1，LIU Juan1   

  1. 1.Key Laboratory of Experimental Teratology, Ministry of Education；Department of Microbiology, School of Medicine；2.Department of Cardiology, Shandong University
  • Received:2007-01-09 Revised:1900-01-01 Online:2007-05-24 Published:2007-05-24
  • Contact: ZHOU Ya-bin

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Abstract: Objective: To study the mechanisms of human cytomegalovirus(HCMV) infection in atherosclerosis by investigating changes of reactive oxygen species (ROS) and sequential changes of the receptor of advanced glyca-Methods： Vascular endothelial cells were cultured and then infected with HCMV, then changes of reactive oxygen species were identified by confocal microscopy; Expression of RAGEmRNA was determined by the reverse transcriptase polymerase chain reaction. Results: Fluorescence intensity was determined at a low level in the control group and the BSA group, with no significant difference (P>0.05). However, it was obviously higher in the AGEs group and the HCMV group than in the control group (P<0.01) and it was further increased in the HCMV combined with AGEs group, which was significantly higher than that in the AGEs and the HCMV groups (P<0.01). RAGEmRNA was expressed at a low level in the control group and the BSA group, with no significant difference (P>0.05). However, it was obviously higher in the HCMV group and the AGEs group than in the control group (P<0.01), and was further increased in the HCMV combined with AGEs group, which was significantly higher than that in the AGEs group and the HCMV group (P<0.01). RAGE mRNA expression gradually increased by an increase of AGEs concentration with the same trites of HCMV in a concentration-dependent manner and was also gradually enhanced by an increase of HCMV trite with the same concentration of AGEs in a trite-dependent manner. Conclusions： Both HCMV infection and AGEs can enhance the oxidative stress and the expression of RAGEmRNA in vascular endothelial cells in a co-effects manner. Both HCMV and AGEs have an effect on the injury of vascular endothelial cells by enhancing the oxidative stress and up-regulating the RAGE expression on endothelial cells, which further facilitates the occurrence and development of atherosclerosis.

Key words: Human cytomegalovirus, Advanced glycation end products, Vascular endothelial cells, Reactive oxygen species, Receptor

CLC Number: 

  • R373
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