JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Gene expression regulation between tongue squamous cell carcinoma cell line Tca-8113 and normal tongue mucosa fibroblasts

WANG Zhen-guang, SUN Shan-zhen, SHI Lin, WANG Dong-guan   

  1. Institute of Pathology，School of Stomatology， Shandong University， Jinan 250012， Shandong， China
  • Received:2006-06-18 Revised:1900-01-01 Online:2007-05-24 Published:2007-05-24
  • Contact: WANG Zhen-guang

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Abstract: Objective： To study the gene expression changes of tongue epithelial tumor cell line Tca-8113 and its neighboring fibroblast (normal mucosa fibroblasts NMFs) after co-culture. Methods： Primarily cultured NMFs were co-cultured with Tca-8113. Then, morphological changes were produced. Changes of growth factors (IGFBP1), signal transducers (Notch3), proteasea/proteases inhibitors (ST3，TIMP3，TFPI-2), and extracellular matrix components or associated protein relating to matrix degradation (Tenascin-C) isolated from oral cancer cells， normal mucosa fibroblast and co-culture cells were determined by semiquantiative RT-PCR experiment. Results: After being co-cultured, NMFs grew surrounding Tca-8113. The cellular organs of the two cells grew more than before co-culture. IGFBP1 of Tca-8113 was decreased, however, TFPI-2 of Tca-8113 or Notch3, TIMP-3, ST3, Tenascin-C of NMFs were increased. Conclusion: The potential complex regulation of Tca-8113 and NMFs raises a question concerning the global pro-or anti-tumor consequences of these heterotypic interactions.

Key words: Tongue neoplasms, Fibroblasts, Gene expression regulation

CLC Number: 

  • R739.85
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