JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• Articles • Previous Articles     Next Articles

Antiproliferation and apoptosis inducing of cyclooxygenase2(Cox-2)

YAN Shu-in,LIU Jia-JUN,SUN Jian-zhi,PENG Jun,LIU Chuan-fang   

  1. Department of Hematology, 1.Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
  • Received:2005-09-14 Revised:1900-01-01 Online:2006-09-24 Published:2006-09-24
  • Contact: YAN Shu-in

Abstract: To investigate the effect of cyclooxygenase2 (Cox2) inhibitor on leukemia HL60 (human acute promylocytic leukeamia cell line) cells. Methods: HL60 cells in culture medium were treated with different concentrations of Cox2 inhibitor, Celecoxib in vitro. MTT assay was used to detect the cell inhibition effect, and flow cytometry (FCM) was used to explore the cell apoptosis. The morphology of cell apoptosis was observed by Hoechst 33258 staining, and the activity of Caspase3 was detected before and after apoptosis. Results: Celecoxib revealed significant antiproliferation and apoptosis inducing effects on HL60 cells in vitro, and the activity of Caspase3 was upregulated remarkably in time and dosedependent manner. Conclusion: Celecoxib has significant antiproliferation and apoptosis inducing effects on HL60 cells, which may closely relate to the increasing of Caspase

Key words: Cyclooxygenase2, Caspases3, Apoptosis

[1] LU Xiang-Dong, YANG Wei, XU Guang-Meng, QU Yuan-Ming. Expression and role of PPAR-γin meningiomas and troglitazone induced meningiomas cell apoptosis in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 65-.
[2] CHEN Yang, FENG Ying, LU Xiao, ZHENG Rong. Galectin-3 promotes autophagy and polarization of macrophages via the PI3K/Akt/mTOR pathway [J]. Journal of Shandong University (Health Sciences), 2026, 64(4): 14-22.
[3] YIN Yue, MO Zhenfei, WU Peixin, LIU Jinxia, WEI Yuanhui, REN Jiabo, LI Chunsun. Expression of GPX1 in lung cancer cell lines and its influence on the proliferation, migration, invasion abilities and apoptosis in lung adenocarcinoma cell lines [J]. Journal of Shandong University (Health Sciences), 2026, 64(1): 65-73.
[4] LI Xiang, ZHANG Yi, WANG Xuechun, XU Mengchao, WANG Yuelan. Research progress on oxidative stress in acute lung injury induced by traumatic brain injury [J]. Journal of Shandong University (Health Sciences), 2025, 63(2): 118-124.
[5] ZHANG Jie, ZHANG Fangfang, WANG Jingnan, LI Zeyu, SONG Ying, LI Na. Expression of circ_0000144 in breast cancer and its effect on the proliferation, migration and invasion ability of breast cancer cells [J]. Journal of Shandong University (Health Sciences), 2025, 63(1): 35-42.
[6] DU Xueshi, NI Xiangmin, LIANG Xinyu, BAI Qian, ZHU Wenyi, WANG Jian. The protect effect of equol and its potential targets in the context of diabetic nephropathy [J]. Journal of Shandong University (Health Sciences), 2024, 62(8): 49-58.
[7] JIANG Zihan, LU Xingchen, SUN Lu, ZHAO Huichen, ZUO Dan, MA Xiaoli, LIU Yuantao, ZHANG Yuchao. Mechanism of NR4A1 regulating hydrogen peroxide-induced apoptosis in human umbilical vein endothelial cells via the IκBα/NF-κB pathway [J]. Journal of Shandong University (Health Sciences), 2024, 62(3): 11-19.
[8] CAO Hualin, JIA Yanzhao, QU Li, YIN Xin. Impacts of CircFAT1 on the proliferation, apoptosis and radiosensitivity of nasopharyngeal carcinoma cells by regulating miR-296-3p/MAPRE1 axis [J]. Journal of Shandong University (Health Sciences), 2023, 61(9): 38-46.
[9] GAO Yujie, LONG Qifu, HU Ying, XU Yuzhen, WANG Ru, YONG Sheng. Bioinformatics identification of the Hub genes and mechanism of hypoxia-induced mitochondrial damage in mouse kidney [J]. Journal of Shandong University (Health Sciences), 2023, 61(9): 57-68.
[10] LIU Jinbo, LIU Kaiwen, XIANG Chongxin, CHENG Lei. Protective effects of crocin on intervertebral disc degeneration [J]. Journal of Shandong University (Health Sciences), 2023, 61(9): 84-93.
[11] ZHANG Jiaying, SU Rongyun, WANG Yinghui, WANG Honggang, LIU Gang. ACE2 gene protects against renal ischemia-reperfusion injury by regulating the Nrf2/HO-1 signaling pathway [J]. Journal of Shandong University (Health Sciences), 2023, 61(4): 1-9.
[12] YANG Yuanfeng, XIONG Gaocai, LI Yuchuan, LUO Yuling, ZHANG Jingjie. Effects of Luling Anshen Granule on the inflammatory response and cell apoptosis in rats with chronic renal failure [J]. Journal of Shandong University (Health Sciences), 2023, 61(10): 9-16.
[13] ZHAO Kai, YIN Xinbao, ZHANG Zongliang, WANG Zhenlin, ZHU Guanqun, WANG Ke. Inhibitory effect and mechanism of astragaloside Ⅱ on renal clear cell carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2023, 61(1): 10-16.
[14] ZHAO Ge, ZOU Cunhua, SONG Dongdong, ZHAO Shuping. Effects of tanshinone IIA on the proliferation and apoptosis of endometrial carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2022, 60(9): 53-58.
[15] LIU Min, ZHANG Yuchao, MA Xiaoli, LIU Xinyu, SUN Lu, ZUO Dan, LIU Yuantao. Effects of orphan nuclear receptor NR4A1 on H2O2 induced injury of mouse renal podocytes [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 16-21.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd