JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (9): 41-46.doi: 10.6040/j.issn.1671-7554.0.2014.716

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Protective effect of grape seed proanthocyanidins in mouse fatty liver models suffering ischemia-reperfusion injury

LI Shaowei1,2,3, LIU Zongzheng1,2, LIU Chunxia1,2, ZHANG Yanru1,2, ZHOU Huanmin1,2   

  1. 1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia, China;
    2. Inner Mongolia Key Laboratory of Biomanufacture, Hohhot 010018, Inner Mongolia, China;
    3. Center of Molecular Biology, Basic Medical College, Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia, China
  • Received:2014-10-16 Online:2015-09-10 Published:2015-09-10
  • Contact: 周欢敏。E-mail:huanminzhou@263.net E-mail:huanminzhou@263.net

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Abstract: Objective To explore the protective effects of grape seed proanthocyanidins (GSP) on liver ischemia-reperfusion (IR) in mouse fatty liver models and the possible mechanism. Methods Thirty C57BL/6 mice were randomly divided into 3 groups: sham-operated group (SO group), IR group (mice with steatotic liver treated with distilled water by gavage just before ischemia reperfusion) and GSP group (mice with steatotic liver gavaged with 500 mg/kg GSP). The mice were then sacrificed 6h after reperfusion, and serum and liver samples were collected. The ALT and AST levels in serum were determined and histiological damage on the liver was evaluated. The infiltration of F4/80 positive macrophage cells was detected using immunohistochemistry. The mRNA expressions of TNF-α, IL-6, iNOS, and INF-γ were assayed using real-time reverse transcription PCR. The expression of p38 protein was detected using Western blotting. Results Compared with the IR group, mice in the GSP group exhibited lower levels of serum ALT and AST (P<0.05), and milder histological damage. Compared with the IR group, mice in the GSP group had reduced infiltration of macrophages and lower mRNA expressions of TNF-α, IL-6, iNOS and INF-γ in the liver tissues (P<0.05). The p38 expressions in the three groups had no difference, but the phosphorylated p38 (p-p38) expression in the GSP group was statistically higher than in the other two groups. Conclusion GSP can alleviate steatotic liver IR injury by inhibiting oxidant stress and inflammatory response induced by reperfusion.

Key words: Inflammatory, Grape seed proanthocyanidins, Oxidant stress, Reperfusion injury, Fatty liver

CLC Number: 

  • R574
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