Journal of Shandong University (Health Sciences) ›› 2026, Vol. 64 ›› Issue (5): 10-18.doi: 10.6040/j.issn.1671-7554.0.2025.0931

• Featured Topics—Bioactive Natural Products • Previous Articles     Next Articles

Isolation, identification, and activity evaluation of secondary metabolites from a fungus Penicillium camemberti RD-37 derived from saline-alkaline soil of the Yellow River Delta

LYU Wenyuan1,2, SI Jie3, REN Siqi1,2, ZHANG Xiaoqi2, FANG Zhou1,2, MIAO Shuang2, GONG Kaikai1,2   

  1. 1. Oncology Department, Binzhou Medical University Hospital, Binzhou 256603, Shandong, China;
    2. Medical Research Center, Binzhou Medical University Hospital, Binzhou 256603, Shandong, China;
    3. Department of Pharmacy, Binzhou Rongjunyoufu Hospital, Binzhou 256600, Shandong, China
  • Online:2026-05-13 Published:2026-05-13

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Abstract: Objective To analyze the secondary metabolites and biological activities of the fungus Penicillium camemberti RD-37, which was isolated from the saline-alkaline soil of the Yellow River Delta. Methods The fermentation extract of this particular fungal strain was isolated and purified through integrated application of separation techniques, including chemical extraction, thin layer chromatography(TLC), octadecylsilyl column chromatography(ODS), and semi-preparative high performance liquid chromatography(HPLC). The structures of the compounds were determined using spectroscopic techniques such as nuclear magnetic resonance(NMR)and mass spectrometry, combined with a comparison of the literature data. The anti-tumor and anti-inflammatory activities of pure compounds were evaluated using CCK-8 and Griess assays. Results Eleven compounds were isolated and identified from the ethyl acetate extract of the rice fermentation of the fungus Penicillium camemberti RD-37, including 6-(methyl 3-methylbutanoate)-7-hydroxy-5-methoxy-4-methylphthalan-1-one(1), 7-hydroxy-5-methoxy-4-methyl-6-(3-methyl-4-oxopentyl)-phthalan-l-one(2), F01-1358B(3), Methyl mycophenolic acid(4), 6-((2E, 6E)-3,7-dimethyldeca-2, 6-dienyl)-7-hydroxy-5-methoxy-4-methylphtanlan-1-one(5), 5,7-dihydroxy-4-methylisobenzofuran-1(3H)-one(6),(2-hydroxypropan-2-yl)-6-methyl-2,3-dihydrobenzofuran-4-ol(7), Radstrictin K(8), 2,3-dihydro-2-hydroxy-2, 4-dimethyl-5-trans-propenylfuran-3-one(9), Benzoic acid(10), p-methoxybenzoic acid(11). The activity results indicated that compound 4(Methyl mycophenolic acid)exhibited significant cytotoxic activities against both human colorectal adenocarcinoma cells RKO and human ovarian cancer cells A2780 cells, with IC50 values of 7.7 μmol/L and 8.2 μmol/L, respectively.In the LPS-induced inflammation model, at a concentration of 30 μmol/L, compounds 1, 6, 8, 9, 10 and 11 exhibited anti-inflammatory activities in mouse mononuclear macrophages cells RAW264.7, with NO inhibition rates of 45.9%, 54.0%, 62.3%, 96.0%, 59.5%, and 55.8%, respectively. Conclusion The fungus Penicillium camemberti RD-37 has the potential to produce compounds with antitumor and anti-inflammatory activities. Compound 4 exhibits significant inhibitory activity against tumor cells.

Key words: Yellow River Delta, Fungi, Penicillium camemberti RD-37, Secondary metabolites, Cytotoxic activity, Anti-inflammatory activity

CLC Number: 

  • R93
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