JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (10): 29-34.doi: 10.6040/j.issn.1671-7554.0.2014.401

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Expression changes of methionine sulfoxide reductase B1 in the kidneys of instreptozocin-induced diabetic mice and its relationship with oxidative stress

YANG Zhiying, LIU Xiangchun, GUAN Guangju   

  1. Nephrology Research Institute, Shandong University;
    Nephrology Department, the Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2014-06-18 Revised:2014-09-19 Online:2014-10-10 Published:2014-10-10

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Abstract: Objective To determine the expression changes of methionine sulfoxide reductase B1 (MsrB1) in the kidneys of streptozotocin (STZ)-induced diabetic mice and to investigate its relationship with oxidative stress. Methods Ten-week-old male C57BL/6 mice were randomly divided into four groups: normal control mice group (NC group), unilaterally nephrectomized mice group (UX group), STZ-induced diabetic mice group (STZ group) and STZ mice with unilateral renal ablation group (STZ-UX group). At the end of the 8th week after the construction of the model, immunohistochemistry detected MsrB1 expression and distribution in the kidney tissues. The mRNA and protein levels of MsrB1 were determined by real-time PCR and Western blotting. The levels of oxidative stress in the kidneys of four groups were measured by the kits of malondialdehyde (MDA), protein carbonyl (PC) and total sulfhydryl groups (TSH). Results MsrB1 was located in the nucleus and cytoplasm of the renal tubular epithelial cells in mice. Compare with NC group, the mRNA and protein levels of MsrB1 and the content of TSH in the kidneys of STZ group and STZ-UX group were lower, the contents of MDA and PC were higher (P<0.05) and STZ-UX group was more obvious; there was no significant change in UX group (P>0.05). Correlation analysis showed that in the STZ group and STZ-UX group, MsrB1 protein expressions were negatively correlated with MDA and PC (P<0.05), and positively correlated with TSH levels (P<0.05). Conclusion The expression of MsrB1 decreases significantly in the kidneys of STZ-induced diabetic mice, which may play an important role in the oxidative stress of diabetic nephropathy.

Key words: Methionine sulfoxide reductase B1, Oxidative stress, C57BL/6 mice, Diabetic nephropathy

CLC Number: 

  • R692
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