JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (10): 25-28.doi: 10.6040/j.issn.1671-7554.0.2014.144

Previous Articles     Next Articles

Effects of azithromycin and dexamethasone on the secretion of ICAM-1 and TNF-α in A549 cells stimulated with Aspergillus fumigatus conidia

WU Fengjuan1, LI Tao2, QI Qian3, LI Yu2   

  1. 1. Department of Pulmonary Disease, Heze Municipal Hospital, Heze 274007, Shandong, China;
    2. Department of Pulmonary Disease, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    3. School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-03-17 Revised:2014-06-10 Online:2014-10-10 Published:2014-10-10

PDF (PC)

306

Abstract: Objective To evaluate the effect of azithromycin and dexamethasone on the secretion of ICAM-1 and TNF-α in A549 cells stimulated with Aspergillus fumigatus conidia. Methods The concentrations of ICAM-1 and TNF-α were detected by ELISA when A549 cells were cultured alone and were cultured with Aspergillus fumigatus conidia for 4 and 24 hours. The normal cultured A549 cells were taken as control group. After being cultured with conidia for 24 hours, A549 cells were treatd with azithromycin (0.5, 2.5 mg/L) or dexamethasone (0.5, 2.5 mg/L) and then the ICAM-1 and TNF-α concentrations were detected by ELISA. Results After being cultured with Aspergillus fumigatus conidia for 4 and 24 hours, both ICAM-1 and TNF-α secreted in A549 cells significantly increased(P<0.05), compared with control group. Compared with the stimulation of Aspergillus fumigatus conidia alone, 2.5 mg/L azithromycin significantly inhibited the secretion of TNF-α in A549 cells incubated with Aspergillus fumigatus conidia for 24 hours(P<0.05). While, 2.5 mg/L dexamethasone significantly inhibited the secretion of both TNF-α (P<0.05) and ICAM-1 in A549 cells incubated with Aspergillus fumigatus conidia for 24 hours (P<0.05). Conclusion Azithromycin and dexamethasone can inhibit the secretion of ICAM-1 and TNF-α in A549 cells stimulated with Aspergillus fumigatus conidia and thus relieve the inflammatory response of lung.

Key words: Cytokines, Azithromycin, Dexamethasone, Aspergillus fumigatus conidia, A549 cells

CLC Number: 

  • R379.6
[1] 中华内科杂志编委会. 侵袭性肺部真菌感染的诊断标准与治疗原则(草案)[J]. 中华内科杂志, 2006, 45(8):697-702.
[2] Balloy V, Sallenave J M, Wu Y, et al. Aspergillus fumigatus-induced interleukin-8 synthesis by respiratory epithelial cells is controlled by the phosphatidylinositol 3-kinase, p38 MAPK and ERKl/2 pathways and not by the toll-like receptor-MyD88 pathway[J]. J Biol Chem, 2008, 283(45):30513-30521.
[3] Bellanger A P, Millon L, Khonfache K, et al. Aspergilhs fumigatus germ tube growth and not conidia ingestion induces expression of inflammatory mediator genes in the human lung epithelial cell line A549[J]. J Med Microbiol, 2009, 58(part 2):174-179.
[4] Luther K, Rohde M, Heesemann J, et al. Quantification of phagocytosis of Aspergillus conidia by macrophages using a novel antibody-independent assay[J]. J Microbiol Methods, 2006, 66(1):170-173.
[5] Filler S G, Sheppard D C. Fungal invasion of normally non-phagocytic host cells[J]. PLoS Pathog, 2006, 2(12):e129. doi:10.1371/journal.ppat.0020129.
[6] Wasylnka J A, Moore M M. Aspergillus fumigatus conidia survive and germinate in acidic organelles of A549 epithelial cells[J]. J Cell Sci, 2003, 116(part 8):1579-1587.
[7] Berkova N, Lair-Fulleringer S, Femenia F, et al. Aspergillus fumigatus conidia inhibit tumour necrosis factor-or staurosporine-induced apoptosis in epithelial cells[J]. Int Immunol, 2006, 18(1):139-150.
[8] Chotirmall S H, Al-Alawi M, Mirkovic B, et al. Expand+Aspergillus-associated airway disease, inflammation, and the innate immune response[J]. Biomed Res Int, 2013, 2013: 723129. doi:10.1155/2013/723129.
[9] Brieland J K, Jackson C, Menzel F, et al. Cytokine networking in lungs of immunocompetent mice in response to inhaled Aspergillus fumigatus[J]. Infect Immun, 2001, 69(3):1554-1560.
[10] Femenia F, Huet D, Lair-Fulleringer S, et al. Effects of conidia of various Aspergillus species on apoptosis of human pneumocytes and bronchial epithelialcells[J]. Mycopatholoqia, 2009, 167(5):249-262.
[11] Chiang L Y, Sheppard D C, Gravelat F N, et al. Aspergillus fumigatus stimulates leukocyte adhesion molecules and cytokine production by endothelial cells in vitro and during invasive pulmonary disease[J]. Infect Immun, 2008, 76(8):3429-3438.
[12] Zimmermann G S, Neurohr C, Villena-Hermoza H, et al. Anti-inflammatory effects of antibacterials on human bronchial epithelial cells[J]. Respir Res, 2009, 10: 89. doi: 10.1186/1465-9921-10-89.
[13] Cigana C, Assael B M, Melotti P. Azithromycin selectively reduces tumor necrosis factor alpha levels in cystic fibrosis airway epithelial cells[J]. Antimicrob Agents Chemother, 2007, 51(3):975-981.
[1] ZHANG Jintao, DONG Liang. Airway epithelium and epithelial-derived cytokines in asthma: reflection and outlook [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 1-6.
[2] NIU Yawen, LI Fengjiao. Role of PAR-2 during Aspergillus fumigatus infection in human corneal epithelial cells [J]. Journal of Shandong University (Health Sciences), 2024, 62(12): 96-101.
[3] WU Fei, LI Qingli, XIAO Zhenwei. Causal association between cytokines and chronic kidney disease based on Mendelian randomization [J]. Journal of Shandong University (Health Sciences), 2024, 62(11): 85-95.
[4] LI Huayu, SHI Xiaohan, ZHANG Xinrui, LI Feng. Association between sleep disturbance and inflammatory cytokines in 203 patients with glioma [J]. Journal of Shandong University (Health Sciences), 2022, 60(12): 26-30.
[5] ZHANG Weiwei, HUA Fang, LIANG Chaoshuai, CHU Miaomiao, SUN Jiayi, FRANK Zaucke, XIN Wei. Thyroid stimulating hormone promotes chondrocyte differentiation via anti-inflammatory protein CTRP3 [J]. Journal of Shandong University (Health Sciences), 2022, 60(10): 1-8.
[6] GE Shaohua, DING Tian, LIU Hongrui. Role and regulatory mechanism of type 2 immunity in tissue repair [J]. Journal of Shandong University (Health Sciences), 2021, 59(9): 51-56.
[7] LI Xiangqing, YIN Xin, ZHAO Xuelian, ZHAO Peiqing. Expression and clinical significance of circulating CD56bright subset of NK cells in patients with Parkinsons disease [J]. Journal of Shandong University (Health Sciences), 2021, 59(2): 34-40.
[8] HUANG Zhuqing, WU Xuewei, REN Dongmei. Isolation and identification of phenolic constituents from Viscum coloratum and the antiproliferative effects on A549 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 35-41.
[9] WANG Qingshi, CHEN Yunzhen, LIU Haichun, WU Wenliang, JIAO Guangjun, LI Xiaofeng, XU Daxia. Effects of inflammatory cytokines secreted by nucleus pulposus cells on proliferation and osteogenic ability of posterior longitudinal ligament fibroblasts [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 22-26.
[10] YUAN Bing, LI Ranran, HAN Mingyong. Primary tumor regulates the pulmonary microenvironment in melanoma carcinoma model and facilitates lung metastasis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(11): 13-18.
[11] LI Xiuhua, LI Xiaoli, DUAN Ruisheng, ZHU Meijia, CAO Lili, LI Yanbin, WANG Si, YUE Longtao, MA Qinghai, LIU Fei. Effects of 1,25(OH)2D3 on the induction of immune tolerance in Lewis rats with EAMG [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 5-10.
[12] YANG Yong-rong, LI Jun, LIANG Ye, LI Jin-du, LAN Jia-fu, LI Tian-zi. The study on association of TNF-α-238G/A gene polymorphisms in patients with oral lichen planus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(S1): 10-11.
[13] CHEN Guoling, REN Ran, ZHANG Yinghui, SONG Wei, KAO Xin, ZHANG Han. Significance and effect of emodin on activation of nuclear factor-κB in corneas of keratitis rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 44-47.
[14] LIU Bing1， YU Zhuang1， HOU Xian-peng1， YAO Ru-yong2. Different expressions of EGFR and HER-3 genes in human lung adenocarcinoma cells and pemetrexed-resistant cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 20-23.
[15] LIU Lu1, ZHANG Wen1, CHEN Han-xiang1, ZHENG Lin1, LU Yi2,WANG Hong1， TANG Wei1, ZHAO Wei-ming1. Inflammatory responses of PGRN-deficient peritoneal macrophage to bacterial lipopolysaccharide in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(3): 58-62.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd