Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (9): 38-46.doi: 10.6040/j.issn.1671-7554.0.2023.0140

• Preclinical Medicine • Previous Articles     Next Articles

Impacts of CircFAT1 on the proliferation, apoptosis and radiosensitivity of nasopharyngeal carcinoma cells by regulating miR-296-3p/MAPRE1 axis

CAO Hualin1, JIA Yanzhao2, QU Li1, YIN Xin1   

  1. 1. Department of Otolaryngology, Head and Neck Surgery;
    2. Radiotherapy Department, Nanyang Central Hospital, Nanyang 473000, Henan, China
  • Received:2023-02-15 Published:2023-10-10

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Abstract: Objective To explore the impacts of circular RNA fat atypical cadherin 1(CircFAT1)on the proliferation, apoptosis and radiosensitivity of nasopharyngeal carcinoma cells by regulating miR-296-3p/microtubule-associated protein RP/EB family member 1(MAPRE1)axis. Methods The expressions of CircFAT1, miR-296-3p and MAPRE1 in NP69, CNE2 and CNE2-RR cells were detected with real-time fluorescent quantitative PCR and Western blotting. CNE2 cells cultured in vitro were randomly divided into the control group, CircFAT1 knockdown group, negative control group, and CircFAT1 knockdown+miR-296-3p inhibitor group. After transfection, the expressions of CircFAT1, miR-296-3p and MAPRE1 were detected with real-time fluorescent quantitative PCR and Western blotting. The proliferation and apoptosis of CNE2 cells in each group were detected with CCK-8 assay, 5-Ethynyl-2’-deoxyuridine_(Edu)staining and flow cytometry. CNE2-RR cells cultured in vitro were randomly divided into the control group, radiation group, radiation+negative control group, radiation+CircFAT1 knockdown group, and radiation+CircFAT1 knockdown+miR-296-3p inhibitor group. After transfection, the expressions of CircFAT1, miR-296-3p and MAPRE1 were detected with real-time fluorescent quantitative PCR and Western boltting; the proliferation and apoptosis of CNE2-RR cells in each group were detected with CCK-8 assay, Edu staining and flow cytometry. The targeting regulation of CircFAT1 on miR-296-3p and miR-296-3p on MAPRE1 were detected with double luciferase reporter gene assay. Results Compared with NP69 cells, CNE2 and CNE2-RR cells had increased expression of CircFAT1 and mRNA and protein expressions of MAPRE1(P<0.05), but decreased expression of miR-296-3p(P<0.05). Compared with CNE2 cells, CNE2-RR cells had increased mRNA and protein expressions of CircFAT1 and MAPRE1(P<0.05), but decreased expression of miR-296-3p(P<0.05). Compared with the control group, the CircFAT1 knockdown group had decreased mRNA and protein expressions of CircFAT1 and MAPRE1, and reduced survival rate and proliferation rate(P<0.05), but increased expression of miR-296-3p and apoptosis rate(P<0.05). There were no significant changes in the indexes of the negative control group(P>0.05); miR-296-3p inhibitor reversed the effects of CircFAT1 knockdown on the indexes. Compared with the control group and radiation group, the radiation + CircFAT1 knockdown group had decreased mRNA and protein expressions of CircFAT1 and MAPRE1, and reduced survival rate and proliferation rate(P<0.05), but increased expression of miR-296-3p and apoptosis rate(P<0.05). There were no significant changes in the cell indexes of the radiation group and radiation + negative control group(P>0.05); miR-296-3p inhibitor reversed the effects of CircFAT1 knockdown on the indicators of CNE2-RR cells under radiation treatment. CircFAT1 targeted and downregulated the expression of miR-296-3p, and miR-296-3p targeted and downregulated the expression of MAPRE1 in CNE2-RR cells. Conclusion Knockdown of CircFAT1 can reduce the expression of MAPRE1 by upregulating miR-296-3p, thereby inhibiting the proliferation, promoting the apoptosis, and enhancing the radiosensitivity of nasopharyngeal carcinoma cells.

Key words: Circular RNA fat atypical cadherin 1, Microtubule-associated protein, Nasopharyngeal carcinoma, Proliferation, Apoptosis, Radiosensitivity

CLC Number: 

  • R739.63
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