Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (9): 19-28.doi: 10.6040/j.issn.1671-7554.0.2023.0243

• Preclinical Medicine • Previous Articles     Next Articles

Clinical value of serum exosomal LINC02163 as a marker for distant metastasis of colorectal cancer

DONG Yaqi1,2, WANG Xinhui1,2, ZHAO Yinghui1,2,3, WANG Chuanxin1,2   

  1. 1. Department of Clinical Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, Shandong, China;
    2. Shandong Provincial Key Laboratory of Innovation Technology in Laboratory Medicine, Jinan 250033, Shandong, China;
    3. Suzhou Research Institute of Shandong University, Suzhou 215123, Jiangsu, China
  • Received:2023-03-21 Published:2023-10-10

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Abstract: Objective To investigate the correlation between long non-coding RNA(lncRNA)LINC02163 and distant metastasis of colorectal cancer and its biological function, and to evaluate the potential of serum exosomal LINC02163 as a marker for distant metastasis. Methods The differential lncRNAs was screened by analyzing the transcriptome data of colorectal cancer tissues. The clinicopathological parameters and demographic parameters of patients were combined to analyze the expression characteristics of LINC02163. The correlation between LINC02163 and prognosis was investigated by in situ hybridization(ISH)in colorectal cancer microarray. The effects of LINC02163 on the distant metastasis of SW620 cells in vivo was investigated by establishing a tumor metastasis model in nude mice. Serum exosomes were identified with transmission electron microscopy, nanoparticle tracking analysis and Western blotting. The stability of serum exosomal LINC02163 was analyzed with RNase A incubation assay. The receiver operating characteristic(ROC)curve was drawn to evaluate the efficacy of serum exosomal LINC02163 in the identification of distant metastasis of colorectal cancer. Results The high expression of LINC02163 in colorectal cancer was screened by five colorectal cancer tissue transcriptome data. TCGA data analysis demonstrated that the expression of LINC02163 was associated with distant metastasis of colorectal cancer(P<0.05). ISH assay showed that high expression of LINC02163 was associated with poor prognosis(P<0.05). Tumor metastasis model in nude mice revealed that LINC02163 knockdown inhibited the distant metastasis of SW620 cells in vivo(P=0.05). Experiments involving the characterization of exosomes showed that serum exosomes were effectively extracted. RNase A incubation demonstrated the high stability of serum exosomal LINC02163. ROC analysis indicated that the area under the curve of serum exosomal LINC02163 was 0.864 for distant metastatic colorectal cancer. Conclusion LINC02163 is highly expressed in colorectal cancer and is associated with distant metastasis. Serum exosomal LINC02163 may become a marker of distant metastasis.

Key words: Colorectal cancer, Distant metastasis, Exosome, Long non-coding RNA, Marker

CLC Number: 

  • R735.3
[1] Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J]. CA Cancer J Clin, 2021, 71(3): 209-249.
[2] Biller LH, Schrag D. Diagnosis and treatment of metastatic colorectal cancer: a review [J]. JAMA, 2021, 325(7): 669-685.
[3] Rawla P, Sunkara T, Barsouk A. Epidemiology of colorectal cancer: incidence, mortality, survival, and risk factors [J]. Prz Gastroenterol, 2019, 14(2): 89-103.
[4] Li N, Lu B, Luo C, et al. Incidence, mortality, survival, risk factor and screening of colorectal cancer: a comparison among China, Europe, and northern America [J]. Cancer Lett, 2021, 522: 255-268. doi: 10.1016/j.canlet.2021.09.034.
[5] Liang ZX, Liu HS, Wang FW, et al. Correction: lncRNA RPPH1 promotes colorectal cancer metastasis by interacting with TUBB3 and by promoting exosomes-mediated macrophage M2 polarization [J]. Cell Death Dis, 2020, 11(6): 465.
[6] Matboli M, Labib ME, Nasser HE, et al. Exosomal miR-1298 and lncRNA-RP11-583F2.2 expression in hepato-cellular carcinoma [J]. Curr Genomics, 2020, 21(1): 46-55.
[7] Zhang Y, Liu H, Liu X, et al. Identification of an exosomal long non-coding RNAs panel for predicting recurrence risk in patients with colorectal cancer [J]. Aging(Albany NY), 2020, 12(7): 6067-6088.
[8] Guo Q, Tang W, Inagaki Y, et al. Subcellular localization of KL-6 mucin in colorectal carcinoma cell lines: association with metastatic potential and cell morphology [J]. Oncol Rep, 2007, 17(5): 1057-1060.
[9] Wen L, Shi X, He L, et al. Manganese-enhanced MRI for the detection of metastatic potential in colorectal cancer [J]. Eur Radiol Exp, 2017, 1(1): 21.
[10] Depciuch J, Klbowski B, Stec M, et al. Similarities in the general chemical composition of colon cancer cells and their microvesicles investigated by spectroscopic methods-potential clinical relevance [J]. Int J Mol Sci, 2020, 21(5): 1826.
[11] 练磊, 兰平. 国家卫健委中国结直肠癌诊疗规范解读(2020版)— 外科部分 [J]. 临床外科杂志, 2021, 29(1): 10-12. LIAN Lei, LAN Ping. National health commission of China colorectal cancer diagnosis and treatment standard interpretation(2020 edition)-surgical part [J]. Journal of Clinical Surgery, 2021, 29(1): 10-12.
[12] Dzunic M, Andjelkovic-Apostolovic M, Vrbic S, et al. Survival of patients with liver metastases from colorectal cancer treated with bevacizumab and FOLFOX4 [J]. J BUON, 2020, 25(1): 212-219.
[13] Ma ZH, Wang YP, Zheng WH, et al. Prognostic factors and therapeutic effects of different treatment modalities for colorectal cancer liver metastases [J]. World J Gastrointest Oncol, 2020, 12(10): 1177-1194.
[14] Kijima S, Sasaki T, Nagata K, et al. Preoperative evaluation of colorectal cancer using CT colonography, MRI, and PET/CT [J]. World J Gastroenterol, 2014, 20(45): 16964-16975.
[15] Hosseini K, Ranjbar M, Pirpour Tazehkand A, et al. Evaluation of exosomal non-coding RNAs in cancer using high-throughput sequencing [J]. J Transl Med, 2022, 20(1): 30. doi: 10.1186/s12967-022-03231-y.
[16] Shen M, Di K, He H, et al. Progress in exosome associated tumor markers and their detection methods [J]. Mol Biomed, 2020, 1(1): 3. doi: 10.1186/s43556-020-00002-3.
[17] Nie H, Liao Z, Wang Y, et al. Exosomal long non-coding RNAs: emerging players in cancer metastasis and potential diagnostic biomarkers for personalized oncology [J]. Genes Dis, 2021, 8(6): 769-780.
[18] Zhang W, Wang Q, Yang Y, et al. The role of exosomal lncRNAs in cancer biology and clinical management [J]. Exp Mol Med, 2021, 53(11): 1669-1673.
[19] Thakur A, Parra DC, Motallebnejad P, et al. Exosomes: small vesicles with big roles in cancer, vaccine development, and therapeutics [J]. Bioact Mater, 2022, 10: 281-294. doi: 10.1016/j.bioactmat.2021.08.029.
[20] Zheng HT, Shi DB, Wang YW, et al. High expression of lncRNA MALAT1 suggests a biomarker of poor prognosis in colorectal cancer [J]. Int J Clin Exp Pathol, 2014, 7(6): 3174-3181.
[21] Chen C, Luo Y, He W, et al. Exosomal long noncoding RNA LNMAT2 promotes lymphatic metastasis in bladder cancer [J]. J Clin Invest, 2020, 130(1): 404-421.
[22] Gao T, Liu X, He B, et al. Exosomal lncRNA 91H is associated with poor development in colorectal cancer by modifying HNRNPK expression [J]. Cancer Cell Int, 2018, 18: 11. doi: 10.1186/s12935-018-0506-2.
[23] Lee YR, Kim G, Tak WY, et al. Circulating exosomal noncoding RNAs as prognostic biomarkers in human hepatocellular carcinoma [J]. Int J Cancer, 2019, 144(6): 1444-1452.
[24] Brennan K, Martin K, Fitzgerald SP, et al. A comparison of methods for the isolation and separation of extracellular vesicles from protein and lipid particles in human serum [J]. Sci Rep, 2020, 10(1): 1039.
[25] Ma J, Zhang L, Shang A, et al. LINC02163 promotes colorectal cancer progression via miR-511-3p/AKT3 axis [J]. Artif Cells Nanomed Biotechnol, 2020, 48(1): 961-968.
[26] Tian Y, Zhou J, Zou Y, et al. Upregulated long noncoding RNAs LINC02163 and FEZF1-AS1 exert oncogenic roles in colorectal cancer [J]. Anticancer Drugs, 2021, 32(1): 66-73.
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