Abstract: Objective To observe the effects of supplementation of multiple trace elements on the anti-inflammatory and antioxidant functions in rats with high-sucrose diet. Methods A total of 40 male Wistar rats were randomly divided into blank group, trace elements(TE)group, high-sucrose diet(HSD)group and high-sucrose diet + trace elements(HSD+TE)group, with 10 rats in each group. Normal saline or a mixture of 11 trace elements(B, V, Cr, Mn, Fe, Co, Cu, Zn, Se, Sr and Mo)was given daily by gavage to the rats. After 20 weeks, the changes of body weight, tissue weight, serum glucose content, oxidative stress and inflammatory markers were measured. Results High-sucrose diet induced a noticeable increase in body weight and serum glucose content(P<0.05), and significantly caused oxidative stress and inflammation(P<0.05). In addition, the supplementation of trace elements significantly inhibited the increase of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)induced by trace element deficiency and high-sucrose diet(P<0.05), but promoted the secretion of interleukin-4(IL-4)and interleukin-10(IL-10)(P<0.05). The supplementation of trace elements inhibited the decrease of the activities of catalase(CAT), total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px)in serum and spleen(P<0.05), reduced the production of malondialdehyde(MDA)(P<0.05), and increased serum glutathione reductase(GR)and total antioxidant capacity(T-AOC)in serum of rats with high-sucrose diet(P<0.05). Maintaining the balance of trace elements prevented weight gain caused by high-sucrose diet(P<0.05). Conclusion The supplementation of multiple trace elements to rats on a high-sucrose diet has a good effect on the prevention of diabetes, which can effectively control the increase of blood glucose level, enhance the antioxidant capacity, reduce the inflammatory response and prevent the weight gain.

Key words: Trace elements, High-sucrose diet, Oxidative stress, Inflammatory factor, Rat