Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (5): 22-30.doi: 10.6040/j.issn.1671-7554.0.2022.0016

Previous Articles     Next Articles

Evening primrose oil resists oxidative stress in the ovaries of rats with polycystic ovary syndrome

HU Na1,2, SUN Miao1,2, XING Shasha1,2, XU Danxia1,3, HAI Xiaoming1,3, MA Ling1,3, YANG Li4, MIAN Yuchen1,2, HE Rui1,2, CHEN Dongmei3, MA Huiming1,2   

  1. 1. Key Laboratory of Fertility Preservation and Maintenance(Ministry of Education), Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    2. Department of Human Anatomy and Tissue Embryology, School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    3. Ningxia Key Laboratory of Stem Cell and Regenerative Medicine, The General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    4. Experimental Animal Center of Ningxia Medical University, Yinchuan 750004, Ningxia, China
  • Published:2022-06-01

PDF (PC)

158

Abstract: Objective To investigate the effects of evening primrose oil(EPO)on the hormonal, metabolic and oxidation levels in rats with polycystic ovary syndrome(PCOS)induced by high-fat diet combined with letrozole, and to explore the expression of NAD-dependent deacetylase sirtuin-1(SIRT1)/Forkhead box O1(FoxO1)signaling pathway in the ovarian tissue. Methods A total of 72 rats were divided into 6 groups, with 12 as the normal control group, and the other 60 rats were fed high-fat diet(40%)for 8 weeks. In the last 3 weeks of the feeding program, letrozole at a concentration of 1 mg/kg/d was added to prepare PCOS models. After the models were established, the serum hormonal levels in the tail of rats were analyzed, and 2 rats with large deviation were removed from each group. Plus the normal control group, the PCOS models were divided into 5 groups, with 10 rats in each group: PCOS model group, metformin group, EPO low-dose group, EPO medium-dose group, and EPO high-dose group. The serum fasting plasma glucose(FPG), serum insulin(FINS), testosterone(T), luteinizing hormone(LH), follicle stimulating hormone(FSH), superoxide Dismutase(SOD), total antioxidant capacity(T-AOC)and malondialdehyde(MDA)of each group were detected with ELISA and biochemical method. The ovarian morphology was observed with hematoxylin and eosin(HE)staining. The locations and expressions of SIRT1/FoxO1 pathway, SOD1 and glutathione S-transferase(GST), SIRT1 and FoxO1 were detected with immunohistochemistry and Western blotting. Results Compared with the blank control group, the PCOS model group had significantly increased weight, FPG, FINS, T, LH, FSH(P<0.05), significantly decreased SOD and T-AOC(P<0.05), and significantly increased MDA(P<0.01). ELISA results showed that after EPO and metformin treatment, the weight, FPG, FINS and MDA decreased(P<0.05), but SOD increased(P<0.05)compared with the PCOS model group. Compared with PCOS model group, the metformin group, medium-dose and high-dose groups had significantly increased T-AOC(P<0.05), but significantly decreased T and LH(P<0.05). Compared with the PCOS model group, the metformin group and high-dose group had significantly decreased FSH level(P<0.05). Immunohistochemical results showed that SOD1, GST and SIRT1 were positively expressed in granulosa cells, mesenchymal cells, primordial follicles and corpus luteum. Western blotting results showed that compared with the blank control group, the PCOS model group had significantly increased expressions of SIRT1, FoxO1, P -FoxO1, P-FoxO1/FoxO1, SOD1 and GST(P<0.05). Compared with the PCOS model group, the high-dose group had significantly decreased levels of SOD1, GST and P-FoxO1(P<0.05); the medium-dose group had significantly decreased levels of SIRT1 and FoxO1(P<0.05). Conclusion EPO affects the levels of oxidative factors and hormones in the serum of PCOS model rats, inhibits the expression of SIRT1/FoxO1 signaling pathway, enhances the antioxidant capacity of ovarian tissue and improve the oxidative stress.

Key words: Evening primrose oil, SIRT1/FoxO1 signaling pathway, Oxidative stress, Polycystic ovarian syndrome, Rats

CLC Number: 

  • R711.75
[1] Ziaei R, Shahshahan Z, Ghasemi-Tehrani H, et al. Effects of inulin-type fructans with different degrees of polymerization on inflammation, oxidative stress and endothelial dysfunction in women with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled trial [J]. Clin Endocrinol(Oxf), 2022, 9. doi: 10.1111/cen.14712.
[2] Dabravolski SA, Nikiforov NG, Eid AH, et al. Mitochondrial dysfunction and chronic inflammation in polycystic ovary syndrome [J]. Int J Mol Sci, 2021, 22(8): 3923. doi: 10.3390/ijms22083923.
[3] Atteia HH, Alzahrani S, El-Sherbeeny NA, et al. Evening primrose oil ameliorates hyperleptinemia and reproductive hormone disturbances in obese female rats: impact on estrus cyclicity [J]. Front Endocrinol(Lausanne), 2019, 10: 942. doi: 10.3389/fendo.2019.00942.
[4] Mert H, Irak K, Cibuk S, et al. The effect of evening primrose oil(Oenothera biennis)on the level of adiponectin and some biochemical parameters in rats with fructose induced metabolic syndrome [J]. Arch Physiol Biochem, 2020, 27:1-9. doi: 10.1080/13813455.2020.1781900.
[5] Moghadam MY, Nemat-Shahi M, Soroosh D, et al. Effect of evening primrose oil on postoperative pain after appendectomy: a double-blind, randomized, clinical trial [J]. Biomedicine(Taipei), 2020, 10(1): 28-32.
[6] 侯巧妮, 马会明, 相丽, 等.人胎盘间充质干细胞移植对化疗所致卵巢早衰大鼠卵巢功能的影响[J]. 山东大学学报(医学版), 2019, 57(2): 52-60. HOU Qiaoni, MA Huiming, XIANG Li, et al. Effects of human placenta mesenchymal stem cell transplantation on ovarian function in premature ovarian failure in rats [J]. Journal of Shandong University(Health Sciences), 2019, 57(2): 52-60.
[7] 杨张杰, 王宇欣, 陈冬梅, 等. 枸杞籽油对小鼠睾丸支持细胞衰老模型的抗炎作用[J].山东大学学报(医学版), 2020, 58(12): 15-22. YANG Zhangjie, WANG Yuxin, CHEN Dongmei, et al. Effects of Lycium barbarum seed oil(LBSO)on anti-inflammation in the aging Sertoli cells of mice(TM4 cells)[J]. Journal of Shandong University(Health Sciences), 2020, 58(12): 15-22.
[8] Dambala K, Paschou SA, Michopoulos A, et al. Biomarkers of endothelial dysfunction in women with polycystic ovary syndrome [J]. Angiology, 2019, 70(9): 797-801.
[9] Khorshidi M, Zarezadeh M, Moradi Moghaddam O, et al. Effect of evening primrose oil supplementation on lipid profile: a systematic review and meta-analysis of randomized clinical trials [J]. Phytother Res, 2020, 34(10): 2628-2638.
[10] Kolan Ska-Dams E, Boinska J, Socha MW. Adipokine levels and carbohydrate metabolism in patients diagnosed de novo with polycystic ovary syndrome [J]. Qatar Med J, 2021, 2: 34. doi: 10.5339/qmj.2021.34. eCollection 2021.
[11] 韩晓婷, 于霞, 董来慧, 等. 月见草油对肥胖型不孕女性代谢及肠道菌群的影响[J].山东大学学报(医学版), 2021, 59(2): 48-54. HANXiaoting, YU Xia, DONG Laihui, et al. Effects of evening primrose oil on the metabolism and intestinal flora of obese infertile women [J]. Journal of Shandong University(Health Sciences), 2021, 59(2): 48-54.
[12] Alam F, Syed H, Amjad S, et al. Interplay between oxidative stress, SIRT1, reproductive and metabolic functions [J]. Curr Res Physiol, 2021, 4: 119-124. doi:10.1016/j.crphys.2021.03.002.
[13] Sun Y, Li S, Liu H, et al. Oxidative stress promotes hyperandrogenism by reducing sex hormone-binding globulin in polycystic ovary syndrome [J]. Fertil Steril, 2021, 116(6): 1641-1650.
[14] Xu B, Dai W, Liu L, et al. Metformin ameliorates polycystic ovary syndrome in a rat model by decreasing excessive autophagy in ovarian granulosa cells via the PI3K/AKT/mTOR pathway [J]. Endocr J, 2022, 26. doi: 10.1507/endocrj.EJ21-0480.
[15] Nikmard F, Hosseini E, Bakhtiyari M, et al. The boosting effects of melatonin on the expression of related genes to oocyte maturation and antioxidant pathways: a polycystic ovary syndrome-mouse model [J]. J Ovarian Res, 2022, 15(1): 11. doi: 10.1186/s13048-022-00946-w.
[16] Mousavi R, Alizadeh M, Asghari Jafarabadi M, et al. Effects of melatonin and/or magnesium supplementation on biomarkers of inflammation and oxidative stress in women with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled trial [J]. Biol Trace Elem Res, 2022, 200(3): 1010-1019.
[17] Nasiri N, Moini A, Eftekhari-Yazdi P, et al. Oxidative stress statues in serum and follicular fluid of women with endometriosis [J]. Cell J, 2017, 18(4): 582-587.
[18] Talat A, Satyanarayana P, Anand P. Association of superoxide dismutase level in women with polycystic ovary syndrome [J]. J Obstet Gynaecol India, 2022, 72(1): 6-12.
[19] 陈雨露. SIRT1在多囊卵巢综合征中的作用及机制研究[D]. 郑州:郑州大学, 2017.
[20] He W, Zhang A, Qi L, et al. FOXO1, a potential therapeutic target, regulates autophagic flux, oxidative stress, mitochondrial dysfunction, and apoptosis in human cholangiocarcinoma QBC939 cells [J]. Cell Physiol Biochem, 2018, 45(4): 1506-1514.
[21] Rong Y, Ren J, Song W, et al. Resveratrol suppresses severe acute pancreatitis-induced microcirculation disturbance through targeting SIRT1-FOXO1 axis [J]. Oxid Med Cell Longev, 2021, 20218891544. doi: 10.1155/2021/8891544.
[22] Xu R, Wang Z. Involvement of Transcription factor FoxO1 in the pathogenesis of polycystic ovary syndrome [J]. Front Physiol, 2021, 12: 649295. doi: 10.3389/fphys.2021.649295.
[23] Zhu L, Duan W, Wu G, et al. Protective effect of hydrogen sulfide on endothelial cells through Sirt1-FoxO1-mediated autophagy [J]. Ann Transl Med, 2020, 8(23): 1586. doi: 10.21037/atm-20-3647.
[24] 李卉, 姜朝阳, 刘岩, 等. 组蛋白去乙酰化酶SIRT1调控氧化低密度脂蛋白诱导巨噬细胞凋亡的实验研究[J]. 山东大学学报(医学版), 2022, 60(1): 6-12. LI Hui, JIANG Chaoyang, LIU Yan, et al. The effects and mechanism of histone deacetylase SIRT1 controlled macrophage apoptosis induced by oxidized low density lipoprotein [J]. Journal of Shandong University(Health Sciences), 2022, 60(1): 6-12.
[1] ZHU Lin, HU San-Yuan, ZHANG Guang-Yong, DING Xiang-Jiu. Protection of PGE2 against intestinal mucosa injuries in obstructed jaundice rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 12-.
[2] SUN Tao, ZHANG Dao-Lai, XIE Shan-Shan, WANG Yu-Zhuo, FENG Yu-Xin, XIN Hua. Effect of ethanol on expression of connexin 43 in primary culture of neural precursor cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 20-.
[3] ZHANG Dao-Lai, SUN Tao, XIE Shan-Shan, WANG Yu-Zhuo, ZHAO Ling, FENG Yu-Xin, XIN Hua. Developmental expression of NMDAR1 subunit on cultured cerebral cortical neurons of fetal rats in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 28-32.
[4] YU Haozhi, SHI Guidong, XU Guopeng, JIANG Yunpeng, FENG Shiqing, LIU Xinyu, QI Lei. Research progress of antioxidant carbon dot nanozymes to regulate the neuro-regeneration microenvironment [J]. Journal of Shandong University (Health Sciences), 2026, 64(2): 44-49.
[5] LI Xiang, ZHANG Yi, WANG Xuechun, XU Mengchao, WANG Yuelan. Research progress on oxidative stress in acute lung injury induced by traumatic brain injury [J]. Journal of Shandong University (Health Sciences), 2025, 63(2): 118-124.
[6] ZHAO Zihui, WANG Xuyang, ZHANG Peng, FANG Ningning, SHI Duanbo, YANG Fan, YANG Xiaomei, WU Jianbo. Rapid and accurate assessment of pulmonary hypertension in rats via diaphragmatic puncture method [J]. Journal of Shandong University (Health Sciences), 2024, 62(2): 20-28.
[7] HU Yanwen, ZHAO Huichen, MA Xiaoli, LIU Yuantao, ZHANG Yuchao. GLP-1 inhibits oxidative stress damage through cytochrome P450 surface oxidase pathway [J]. Journal of Shandong University (Health Sciences), 2023, 61(8): 10-16.
[8] YAN Congcong, CHEN Chen, XIE Qian, WANG Yanan, ZHANG Xinlu, ZHANG Yingchun, WU Bin. Effects of bisphenol A exposure on m6A modification level of KGN cells [J]. Journal of Shandong University (Health Sciences), 2023, 61(8): 17-23.
[9] LIU Yang, CHEN Guihai. Effects and mechanism of Hanjingtang on the oxidative stress induced by cold stimulation in aortic vascular smooth muscle cells [J]. Journal of Shandong University (Health Sciences), 2023, 61(8): 24-30.
[10] QI Shaojun, TANG Yanjin, ZHANG Zhengduo, WU Hong, ZHANG Jiacheng, QIN Chuan, LIU Rui, GAO Xibao. Protective effects of supplementing various trace elements on rats with high-sucrose diet [J]. Journal of Shandong University (Health Sciences), 2023, 61(7): 19-26.
[11] ZHANG Jiaying, SU Rongyun, WANG Yinghui, WANG Honggang, LIU Gang. ACE2 gene protects against renal ischemia-reperfusion injury by regulating the Nrf2/HO-1 signaling pathway [J]. Journal of Shandong University (Health Sciences), 2023, 61(4): 1-9.
[12] WU Hong, ZHANG Zhengduo, TANG Yanjin, QI Shaojun, GAO Xibao. Potential intervention effects of 5-methyltetrahydrofolate on atherosclerosis in rats [J]. Journal of Shandong University (Health Sciences), 2022, 60(8): 6-13.
[13] LIU Min, ZHANG Yuchao, MA Xiaoli, LIU Xinyu, SUN Lu, ZUO Dan, LIU Yuantao. Effects of orphan nuclear receptor NR4A1 on H2O2 induced injury of mouse renal podocytes [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 16-21.
[14] ZHAO Huiwen, XU Lin, SHAN Shan, ZHAO Xiulan. Protective effect of taurine on cognitive deficit induced by 1-bromopropane [J]. Journal of Shandong University (Health Sciences), 2022, 60(2): 14-21.
[15] HUANG Huining, DU Juanjuan, SUN Yi, HOU Yinglong, GAO Mei. Hydrogen sulfide alleviates acute obstructive sleep apnea-induced atrial fibrillation by regulating oxidative stress through glutaredoxin-1 [J]. Journal of Shandong University (Health Sciences), 2022, 60(1): 1-5.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd