Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (11): 53-58.doi: 10.6040/j.issn.1671-7554.0.2019.1426

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Effects of propofol on proliferation, migration and expressions of Wnt1 and β-catenin in colon cancer cells

HONG Jiageng1, NIE Yangyang1, SU Guoqiang2   

  1. 1. Department of Anesthesiology;
    2. Department Ⅲ of Gastrointestinal Surgery, First Affiliated Hospital of Xiamen University, Xiamen 361003, Fujian, China
  • Published:2020-11-04

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Abstract: Objective To investigate the effects of propofol on the proliferation, migration and mRNA expressions of Wnt1 and β-catenin in human colon cancer cells with different degrees of differentiation in vitro. Methods Two human colon cancer cell lines Caco-2(highly differentiated)and LoVo(lowly differentiated)were selected and assigned to P0(0 μg/mL), P6.25(6.25 μg/mL), P25(25 μg/mL), and P100(100 μg/mL)groups according to different concentrations of propofol. The effects of propofol on the proliferation and migration of Caco-2 and LoVo cells were detected with CCK-8 and Transwell migration assay, respectively. The sensitivity of cells to different concentrations of propofol was compared. The mRNA expressions of Wnt1 and β-catenin in Caco-2 and LoVo cells were determined with real-time PCR. Results The CCK-8 results showed significant difference in Caco-2 viability between P100 group treated for 24 h and P0 group(P<0.001), and between P25 group treated for 48 h and P0 group(P<0.05); and significant difference in LoVo viability between P100 group treated for 24 h and P0 group(P<0.05 ), and between P25 group treated for 48 h and P0 group(P<0.05). Transwell migration results showed significant difference in OD570 nm value in Caco-2 cell line between P6.25, P25, P100 groups and P0 group(P<0.001); and in LoVo cell line between P25, P100 groups and P0 group(P<0.001). Real-time PCR results showed significant difference in Wnt1 mRNA expression in Caco-2 cell line between P25, P100 groups and P0 group(P<0.05), in LoVo cell line between P25, P100 groups and P0 group(P<0.001); and significant difference in β-catenin mRNA expression in Caco-2 cell line between P25, P100 groups and P0 group(P<0.001), and in LoVo cell line between P100 group and P0 group(P<0.001). Conclusion Propofol inhibits the proliferation and migration of human colon cancer cells Caco-2 and LoVo in a dose- and time-dependent manner. Caco-2 is more sensitive to the inhibitory effects of propofol than LoVo.

Key words: Propofol, Colorectal cancer, Proliferation, Migration, Wnt/β-catenin signaling pathway

CLC Number: 

  • R735
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