Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (6): 68-74.doi: 10.6040/j.issn.1671-7554.0.2018.1443

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Effect of ar-turmerone on poliferation, migration and invasion of cervical squamous cancer cell line SiHa

LUO Mengying1, WU Bei2, WANG Ye1, LUO Heng3,4, CAO Yu1   

  1. 1. Guizhou Medical University, Guiyang 550004, Guizhou, China;
    2. Department of Gynecology and Obstetrics, the 44 Hospital of Peoples Liberation Army, Guiyang 550009, Guizhou, China;
    3. State Key Laboratory of Functions and Applications of Medicinal Plants, Guiyang 550014, Guizhou, China;
    4. Key Laboratory of Chemistry for Natural Products of Guizhou Province, Chinese Academy of Sciences, Guiyang 550014, Guizhou, China
  • Published:2022-09-27

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Abstract: Objective To investigate the effect of ar-turmerone on the proliferation, migration and invasion of cervical cancer SiHa cells in vitro, and to preliminarily explore its mechanism. Methods SiHa cells were incubated with different concentrations of ar-turmerone(0, 20, 40, 80 mg/L), and cell proliferation was measured by CCK8. The apoptosis was observed by giemsa staining. The effects of ar-turmerone on migration and invasion capacity of SiHa cells were detected by cell scratch assay and transwell. The apoptosis was analyzed by flow cytometry. The expressions of MMP2, MMP9 and p53 protein in SiHa cells were detected by Western blotting. Results Ar-turmerone dose-dependently inhibited SiHa cells proliferation(Fconcentration=162.419, P<0.001; Ftime=99.442, P<0.001; Fconcentration×time=2.111, P=0.095), migration(Fconcentration=432.158, P<0.001; Ftime=528.845, P<0.001)and invasion(Fconcentration=434.362, P<0.001). 山 东 大 学 学 报 (医 学 版)57卷6期 -罗梦颖,等.芳姜黄酮对SiHa细胞增殖、迁移及侵袭的影响 \=-Flow cytometry analysis showed that ar-turmerone could induce the apoptosis of SiHa cells in a dose-dependent manner(Fconcentration=482.690, P<0.001). Western blotting results showed that ar-turmerone treatment significantly up-regulated the expression of apoptosis-related proteins p53(Fconcentration=2.086, P=0.045)and down-regulated the expressions of invasion and metastasis related proteins MMP2 and MMP9(Fconcentration=18.906, P=0.001; Fconcentration=9.847, P=0.005). Conclusion Ar-turmerone has the ability to inhibit the proliferation and induce apoptosis of SiHa cells in vitro, and the mechanism is related to the regulation of apoptosis related protein p53 expression. In addition, ar-turmerone can inhibit the migration and invasion of SiHa cells, possibly through the regulation of the expressions of MMP2 and MMP9 protein.

Key words: Ar-turmerone, SiHa cell, Apoptosis, Migration, Invasion

CLC Number: 

  • R285.5
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