Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (4): 9-14.doi: 10.6040/j.issn.1671-7554.0.2018.1344

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Effects of c-Jun N-terminal kinase regulated by CD40siRNA on the autophagy of cardiomyocytes in rats with autoimmune myocarditis

SUN Honglin1, HAN Bo1, WANG Jing1, GAO Ling2, ZHU Mei1, JIANG Diandong1, LÜ Jianli1   

  1. 1. Department of Pediatric Cardiology;
    2. Central Laboratory, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the effects of CD40siRNA regulated c-Jun N-terminal kinase(JNK)pathway on the autophagy of cardiomyocytes in experimental autoimmune myocarditis(EAM). Methods A total of 32 healthy male Lewis rats aged 6 to 8 weeks were randomly divided into the EAM group, EAM+CD40siRNA group, EAM+siRNA control group, and normal control group(NC group), with 8 rats in each group. The EAM models were established by injecting mixed porcine heart immunoglobulin(0.2 mL/rat)into the hind paw pad of rats in the first 3 groups on day 0 and day 7. Rats in the NC group were injected with 0.2 mL/rat of phosphate buffered saline(PBS). On day 7, rats in the EAM+CD40siRNA group were injected with 25 μL of CD40siRNA lentiviral vector in the tail vein, and rats in the EAM+siRNA control group were injected with 25 μL of LsiRNA lentiviral vector. The body weight of rats was measured every 3 days. All rats were sacrificed on day 21. Pathological changes of the myocardial tissues were observed 山 东 大 学 学 报 (医 学 版)57卷4期 -孙红林,等.CD40siRNA调控c-Jun氨基末端激酶对自身免疫性心肌炎大鼠心肌细胞自噬的影响 \=-with a light microscopy and pathological scores were calculated. The JNK was immunohistochemically stained. The autophagy-related protein Beclin1 and microtubule-associated protein light chain 3-Ⅱ/Ⅰ ratio(LC3-Ⅱ/Ⅰ)were detected with Western blotting. Results During the experiment, no rats died. The body mass of rats in the NC group increased faster than that in the EAM group(P<0.05). The EAM+CD40siRNA group had significantly lower pathological scores than the EAM group(P<0.001). The EAM+CD40siRNA group and NC group had significantly lower LC3-Ⅱ/Ⅰ and Beclin1 than the EAM group, and there was a positive correlation between JNK and LC3-Ⅱ(r=0.985, P<0.001). Conclusion The CD40siRNA can relieve the inflammation of EAM rats, possibly by downregulating the JNK pathway and inhibiting the autophagy of cardiomyocytes.

Key words: Autoimmune myocarditis, Autophagy, CD40-CD40 ligand, C-Jun N-terminal kinase, Microtubule-associated protein light chain 3-II/I

CLC Number: 

  • R725.4
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