JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (8): 6-11.doi: 10.6040/j.issn.1671-7554.0.2015.1171

Previous Articles     Next Articles

miR-1/133 participates in the expression of ion channel genes of myocardial cells in myocarditis mice

LIU Mengmeng, ZHAO Cuifen, KONG Qingyu, CAI Zhifeng, XIA Wei   

  • Received:2015-11-26 Online:2016-08-10 Published:2016-08-10

PDF (PC)

761

Abstract: Objective To explore the involvement of miR-1/133 in regulating the expression of potassium and calcium ion channel in mouse with acute viral myocarditis. Methods After the mice models of acute viral myocarditis were established, they were divided into 4 groups: control group, viral myocarditis group, myocarditis+miR-1/133 mimics group, and myocarditis+miR-1/133 NC group, with 10 mice in each group. The myocardial pathological changes were observed with HE staining. The expressions of miR-1, miR-133, Kcnd2, Irx5, Kcnj2, and α1c in myocardium were detected with qRT-PCR. The expressions of Kv4.2, Kir2.1, and Cav1.2 in myocardium were detected with Western blotting. Results HE staining showed that, in the control group, myocardial cells arranged orderly, and no inflammatory cells infiltrated myocardium matrix; in the myocarditis group and myocarditis+miR-1/133 NC group, myocardial cells swelled and arranged disorderly, and inflammatory cells infiltrated myocardium matrix; in the myocarditis+miR-1/133 mimics group, myocardial cells arranged orderly without cell edema, and few inflammatory cells infiltrated myocardium matrix. Compared with the control group, in the myocarditis group and myocarditis+miR-1/133 NC group, the expressions of miR-1, miR-133, Kcnd2, Kcnj2, Kv4.2 and Kir2.1 in the myocardium significantly decreased(P<0.01), while the expressions of Irx5, α1c and Cav1.2 increased(P<0.01). Compared with the myocarditis 山 东 大 学 学 报 (医 学 版)54卷8期 -刘蒙蒙,等.miR-1/133对病毒性心肌炎小鼠心肌细胞离子通道表达的影响 \=-group and myocarditis+miR-1/133 NC group, in the myocarditis+miR-1/133 mimics group, the expressions of miR-1, miR-133, Kcnd2, Kcnj2, Kv4.2 and Kir2.1 in the myocardium were upregulated(P<0.05), while the expressions of Irx5, α1c and Cav1.2 were downregulated(P<0.01). Conclusion miR-1/133 is involved in the regulation of calcium and potassium ion channel gene expression in mice with acute viral myocarditis.

Key words: Potassium channel, miR-1, miR-133, Viral myocarditis, Calcium channel, Gene expression

CLC Number: 

  • R725.4
[1] Meister G, Tuschl T. Mechanisms of gene silencing by double-stranded RNA[J]. Nature, 2004, 431(7006):343-349.
[2] Alvarez-Garcia I, Miska EA. MicroRNA functions in animal development and human disease[J]. Development, 2005, 132(21):4653-4662.
[3] Miranda KC, Huynh T, Tay Y, et al. A pattern-based method for the identification of MicroRNA binding sites and their corresponding heteroduplexes[J]. Cell, 2006, 126(6):1203-1217.
[4] Takaya T, Ono K, Kawamura T, et al. MicroRNA-1 and MicroRNA-133 in spontaneous myocardial differentiation of mouse embryonic stem cells[J]. Circ J, 2009, 73(8):1492-1497.
[5] Besser J, Malan D, Wystub K, et al. MiRNA-1/133a clusters regulate adrenergic control of cardiac repolarization[J]. PLoS One, 2014, 9(11):113449. doi:10.1371.
[6] Feldman AM, McNamara D. Myocarditis[J]. N Engl J Med, 2000, 343(19):1388-1398.
[7] Rose NR, Herskowitz A, Neumann DA. Autoimmunity in myocarditis:models and mechanisms[J]. Clin Immunol Immunopathol, 1993, 68(2):95-99.
[8] Care A, Catalucci D, Felicetti F, et al. MicroRNA-133 controls cardiac hypertrophy[J]. Nat Med, 2007, 13(5):613-618.
[9] Tao G, Martin JF. MicroRNAs get to the heart of development[J]. Elife, 2013, 2:1710. doi:10.7554.
[10] He B, Xiao J, Ren A J, et al. Role of miR-1 and miR-133a in myocardial ischemic postconditioning[J]. J Biomed Sci, 2011, 18:22.
[11] Sayed D, Hong C, Chen IY, et al. MicroRNAs play an essential role in the development of cardiac hypertrophy[J]. Circ Res, 2007, 100(3):416-424.
[12] Yang B, Lin H, Xiao J, et al. The muscle-specific microRNA miR-1 regulates cardiac arrhythmogenic potential by targeting GJA1 and KCNJ2[J]. Nat Med, 2007, 13(4):486-491.
[13] Wang GK, Zhu JQ, Zhang JT, et al. Circulating microRNA:a novel potential biomarker for early diagnosis of acute myocardial infarction in humans[J]. Eur Heart J, 2010, 31(6):659-666.
[14] 刘玉学, 王欣. 心房颤动中心房重构机制的研究进展[J]. 中国胸心血管外科临床杂志, 2009, 16(3):218-222. LIU Yuxue, WANG Xin. Advances of mechanism of the atrial remodeling in atrial fibrillation[J]. Chin J Clin Thorac Cardiovasc Surg, 2009, 16(3):218-222.
[15] Guo Q, Peng TQ, Yang YZ. Effect of Astragalus membranaceus on Ca2+ influx and coxsackie virus B3 RNA replication in cultured neonatal rat heart cells[J]. Chinese J of Integrated Traditional and Western Med, 1995, 15(8):483-485.
[16] Shan H, Zhang Y, Cai B, et al. Upregulation of microRNA-1 and microRNA-133 contributes to arsenic-induced cardiac electrical remodeling[J]. Int J Cardiol, 2013, 167(6):2798-2805.
[17] Lu Y, Zhang Y, Shan H, et al. MicroRNA-1 downregulation by propranolol in a rat model of myocardial infarction:a new mechanism for ischaemic cardioprotection[J]. Cardiovasc Res, 2009, 84(3):434-441.
[18] Diaz RJ, Zobel C, Cho HC, et al. Selective inhibition of inward rectifier K+ channels(Kir2.1 or Kir2.2)abolishes protection by ischemic preconditioning in rabbit ventricular cardiomyocytes[J]. Circ Res, 2004, 95(3):325-332.
[19] Costantini DL, Arruda EP, Agarwal P, et al. The homeodomain transcription factor Irx5 establishes the mouse cardiac ventricular repolarization gradient[J]. Cell, 2005,123(2):347-358.
[20] Zhao Y, Ransom JF, Li A, et al. Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2[J]. Cell, 2007, 129(2):303-317.
[21] 王玉琴, 耿鹏, 吴扬. miR-1和miR-133a对大鼠肥大心肌细胞L-型钙通道Cavβ_2和α1c亚基的调控作用[J]. 基础医学与临床, 2015, 35(2):196-202. WANG Yuqin, GENG Peng, WU Yang. Regulation of miR-1 and miR-133a on L-type calcium channel Cavβ2 and α1c subunits in rat cardiomyocyte hypertrophy[J]. Basic & Clinical Medicine, 2015, 35(2):196-202.
[1] ZHANG Qiuping, ZHU Huizhi, LYU Chuan, XIA Yongqi, ZHANG Xiu. Identification of potential key autophagy- and ferroptosis-related genes in asthma based on bioinformatics analysis [J]. Journal of Shandong University (Health Sciences), 2026, 64(1): 74-87.
[2] SONG Huishu, SUN Zhijian, ZHANG Qiuting, MI Xue, ZHANG Ni, WANG Zhilun, YIN Zhe, HAO Xuexi, WANG Yingxue. Effet of miR-125a-3p on the proliferation of rheumatoid arthritis synovial fibroblasts [J]. Journal of Shandong University (Health Sciences), 2025, 63(12): 74-78.
[3] JING Kai, XU Zhiwei, LI Leping. Exploring colorectal cancer related genes and their functions based on GEO [J]. Journal of Shandong University (Health Sciences), 2024, 62(1): 21-30.
[4] HAN Xiao, LI Lei, LIU Congcong. Effect of testosterone on the expression of polycystic ovary syndrome susceptibility gene Tox3 in zebrafish [J]. Journal of Shandong University (Health Sciences), 2021, 59(4): 42-47.
[5] YANG Jia, ZHANG Man, CHEN Kaiming, CAO Xi. Mechanism of miR-146a accelerating macrophage migration induced arteriosclerosis via TLR4/MyD88 pathway [J]. Journal of Shandong University (Health Sciences), 2021, 59(11): 1-7.
[6] SUI Rongcui, HAN Shuhui, ZHANG Xianzhao, FAN Xintai, WANG Na, HOU Lingxiao, XU Anting. Localization and expression of L-type calcium channel in mice primary endolymph sac epithelium [J]. Journal of Shandong University (Health Sciences), 2021, 59(10): 18-24.
[7] LI Lintong, HOU Xinguo, QIN Jun, LIANG Kai, REN Jianmin. A case report of maturity-onset diabetes of the young13 [J]. Journal of Shandong University (Health Sciences), 2020, 58(6): 71-75.
[8] JIN Haiyan, ZHANG Yan, MA Xiaoli, HAN Yu, ZHAO Huichen, LIU Yuantao, ZHANG Yuchao. Expressions of miR-122 and miR-33a in patients with type 2 diabetes complicated with coronary artery disease [J]. Journal of Shandong University (Health Sciences), 2020, 58(3): 94-98.
[9] LIU Congcong, LIU Hongbin, FU Jiang, LI Lei. Expression of polycystic ovary syndrome susceptibility gene dennd1a in zebrafish models [J]. Journal of Shandong University (Health Sciences), 2019, 57(1): 48-54.
[10] LIU Jingkang, YANG Jianyong, MENG Lihua, JIANG Jie. The value of serum miR-17-92 cluster in early diagnosis of HPV-positive cervical cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 86-90.
[11] LI Chang, ZHANG Qian, MA Fang, XIE Qi, CHANG Xiaotian. Relationship between PADI2 and genetic susceptibility in various human tumors [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 47-53.
[12] YU Guifang, CHEN Shudi, CHEN Xuezhu, HOU Kailian, LIANG Min. miR-196a promotes cell growth of HBx-HepG2 via targeting SOCS6 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(12): 14-19.
[13] CHEN Yan, LIU Juan, CHEN Hanxiang, ZHANG Weifang, ZHAO Weiming. Effects of miR-17 on the senescence of human foreskin fibroblasts [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 55-59.
[14] ZHANG Yanli, LIU Xinfeng, ZHANG Xin, WANG Haiyan, YANG Yongmei, DU Lutao, WANG Lili, LI Peilong, WANG Chuanxin. Expression of circulating miR-128 in serum of colorectal cancer and its effect on migration and invasion of colorectal cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(8): 57-62.
[15] ZHANG Wen1, FENG Tingting1, ZHENG Lin1, WANG Hong1, LU Yi2, QI Mei1, YU Xiuping1, TANG Wei1, ZHAO Weiming1. Effects of enhanced expression of RbAp48 mediated by recombinant adenovirus on proliferation and growth of cervical cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(4): 13-17.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd