JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (S1): 14-17.

Previous Articles     Next Articles

Effects of Chaipu decoction on expression of VEGF and TGF-β1on airway remodeling in asthmatic rats

HUANG Yan1, KUANG Xin2, Xiong Hua3, LIU Xin1, LUO Xiao-qin3, LIU Bin3   

  1. 1. Department of Traditional Chinese Medicine, the First Hospital Affiliated to University of South China, Hengyang Hunan 421001, China;
    2. Department of Anesthesiology, the First Hospital Affiliated to University of South China, Hengyang Hunan 421001, China;
    3. University of South China, Hengyang Hunan 421001, China
  • Received:2014-11-30 Published:2014-12-30
  • Contact: 旷昕, gdl0422@qq.com E-mail:gdl0422@qq.com

PDF (PC)

148

Abstract: Objective To investigate the machnism of Chaipu decoction in treatment of asthma and its effects on expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). Methods Forty SD rats randomly divided into four groups: the control group (group A), the dexamethasone group (group B), the ChaiPu decoction group (group C), the asthma chronic model group (group D). Airway remodeling was observed by H&E staining. Expression of VEGF, TGF-β1 mRNA and protein were measured by RT-PCR and immune histochemical method, respectively. Results Inflammatory cell infiltration, angiogenesis, thickening of the epithelial basement membrane and bronchial wall and bronchial narrowing were observed in D model group. The results of RT-PCR semi-quantitative analysis showed that gray value of VEGF, TGF-β1 of group D were significantly higher than other groups(P<0.05). The observed value of integral optical density (IOD) of VEGF, TGF-β1 were highest in group D (P<0.01) and lowest in group A (P<0.05 or P<0.01). Conclusion The increased expression of VEGF and TGF-β1 in asthmatic rats suggest that VEGF and TGF-β1 may be involved in airway modulating. Early use of Chaipu decoction in the treatment of asthma may down-regulate VEGF, TGF-β1 expression, therefore inhibit airway remodeling.

Key words: Chaipu decoction, VEGF, TGF-β1, Airway remodeling, Asthma

CLC Number: 

  • R361
[1] Akinbami L J,Moorman J E,Liu X.Asthma prevalence,health care use,and mortality: United States,2005-2009[J].Natl Health Stat Report,2011,(32): 1-14.
[2] Knox A J,Corbett L,Stocks J,et a1.Human airway smooth muscle cells secrete vascular endothelial growth factor: up-regulation by bradykinin via a protein kinase C and prostanoid dependent [J].FASEB J,2001,15(13): 2480-2488.
[3] Lee K S,Min K H,Kim S R,et a1.Vascular endothelial growth factor modulates matrix metalloproteinase-9 expression in asthma [J].Am J Respir Crit Care Med,2006,174(2): 161-170.
[4] Kasperska-Zajac A.Asthma and Dehydroepiandrosterone (DHEA): Facts and Hypotheses[J].Inflammation,2010,33(5): 320-324.
[5] Alrifai M,Marsh LM,Dicke T,et al.Compartmental and temporal dynamics of chronic inflammation and airway remodelling in a chronic asthma mouse model[J].PLoS One,2014 ,9(1): e85839.
[6] 刘鑫,梅全慧,刘希.VEGF、H IF 1 在大鼠慢性哮喘模型中的表达及地塞米松的干预效应[J].重庆医学,2010,39(22): 3013-3015.
[7] Gomulka K,Liebhart J.Vascular endothelial growth factor - structure,function and role in airways inflammation and the clinical course of asthma[J].Pneumonol Alergol Pol,2009,77(6): 549-553.
[8] 李玉梅,安彩霞.转化生长因子-β1与支气管哮喘患者呼吸道重塑的关系研究进展[J].实用儿科医学杂志,2009,24(16): 1279-1281.
[9] 彭光耀,刘鑫,黄艳,等.TGF-β1/Smads通路在哮喘大鼠气道重塑模型中的动态变化[J].广东医学,2010,31(5): 557-560.
[1] ZHANG Qiuping, ZHU Huizhi, LYU Chuan, XIA Yongqi, ZHANG Xiu. Identification of potential key autophagy- and ferroptosis-related genes in asthma based on bioinformatics analysis [J]. Journal of Shandong University (Health Sciences), 2026, 64(1): 74-87.
[2] ZHANG Zheng, WANG Jianwei, YANG Yujuan, ZHANG Yu, SONG Xicheng. Immunoglobulin E changes and risk factors in asthma children between 2008 and 2019 [J]. Journal of Shandong University (Health Sciences), 2025, 63(7): 32-36.
[3] CAO Luofei, WANG Shanshan, WANG Jinrong, JIANG Heyun, MIAO Yu, MA Guangzeng. Analysis of the characteristics of bronchial dilation test in children with FeNO elevation during asthma exacerbation [J]. Journal of Shandong University (Health Sciences), 2025, 63(6): 38-44.
[4] SUN Congcong, CUI Wenjing, ZHANG Jintao, ZHANG Dong, LIU Xiaofei, PAN Yun, QI Qian, XU Jiawei, ZENG Rong, GUO Hongxi, DONG Liang. Roles of ferroptosis in asthmatic airway remodeling [J]. Journal of Shandong University (Health Sciences), 2024, 62(7): 1-9.
[5] WANG Jing, LIU Xiaofei, ZENG Rong, XU Changjuan, ZHANG Jintao, DONG Liang. Identification of necroptosis-related biomarkers in asthma based on machine learning algorithms [J]. Journal of Shandong University (Health Sciences), 2024, 62(7): 21-32.
[6] YAN Jinyan, YANG Chun, LI Lei, WU Fuling, JIAO Yongli, ZHANG Xiaowei, LI Jing, ZHANG Ruizhen, WANG Lei, MA Xiang. Correlation between asthma and pertussis infection in children of Shandong Province, China [J]. Journal of Shandong University (Health Sciences), 2024, 62(7): 33-41.
[7] ZHANG Jintao, DONG Liang. Airway epithelium and epithelial-derived cytokines in asthma: reflection and outlook [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 1-6.
[8] DING Yiren, LIU Wanying, YAO Lei, YAO Xin. Research progress of the treatment of asthma with macrolide antibiotics [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 21-27.
[9] WANG Ting, ZHANG Li, WANG Gang. Neuropsychological asthma [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 28-34.
[10] XU Fang, TIAN Guoxiong, SUN Beibei, CHEN Xinyi, CHEN Gaoying, ZHANG Ruiqi, YING Songmin, WU Miaolian, ZHANG Chao, WU Youqian. Research progress on biological and cellular therapies for severe asthma [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 35-42.
[11] SHI Shuochuan, ZENG Rong, ZHANG Jintao, ZHANG Dong, PAN Yun, LIU Xiaofei, XU Changjuan, WANG Ying, DONG Liang. Bioinformatics-based exploration of potential differential immune genes and immune infiltration signatures in bronchial asthma [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 43-53.
[12] XU Xinjun, SHAO Liting, CHEN Ying, LIU Huifang, YANG Yujuan, ZHANG Yu, WANG Hanrui, SONG Xicheng. Comparison of the therapeutic effect of SYN008 with Xolair® in allergic asthma mice: inflammation and remodeling [J]. Journal of Shandong University (Health Sciences), 2024, 62(12): 1-10.
[13] HAO Yan, CUI Limei, CHEN Ying, YANG Yujuan, SONG Xicheng. Advances of metabolomics in airway inflammatory diseases [J]. Journal of Shandong University (Health Sciences), 2024, 62(12): 117-124.
[14] BU Meiling, WANG Jinrong, FENG Mei, SUN Lifeng. Mechanism of FOXM1 in acute exacerbation of asthma induced by respiratory virus infection in mice [J]. Journal of Shandong University (Health Sciences), 2023, 61(6): 1-9.
[15] ZHANG Qian, QIN Mingming, HE Xuejia, CAI Qiujing, ZHANG Yamin, LI Qingsu, ZHU Weiwei. Effects of calcitriol on EMT induced by TGF-β1 in asthma [J]. Journal of Shandong University (Health Sciences), 2021, 59(7): 10-18.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd