JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 26-29.doi: 10.6040/j.issn.1671-7554.0.2014.236

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Impact of aging on the nigro-strital oxidative stress in a mice model of Parkinson's disease

ZHANG Zhongxia, MA Xiaowei, WANG Yanyong, LI Xiaoli, WANG Mingwei   

  1. Department of Neurology, the First Hospital of Hebei Medical University, Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang 050031, Hebei, China
  • Received:2014-03-21 Revised:2014-06-23 Online:2014-09-10 Published:2014-09-10
  • Contact: 王铭维。E-mail:wmw@hebmu.edu.cn E-mail:wmw@hebmu.edu.cn

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Abstract: Objective To observe the age-related changes of nigro-strital oxidative stress in a mice model of Parkinson's disease(PD) at different ages and detect the differential expression of oxidative stress related genes in aged PD mice by using PCR Array. Methods Forty-two healthy female senescence-accelerated mouse prone 8 (SAMP8) mice aged 3, 6 and 10-month were averagely and randomly divided into 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) group and control group, which were subcutaneously injected with MPTP or the same volum of 0.9% NaCl, respectively. After the first injection for 72 hours, behavioral changes in mice were examined by the open field test. The levels of dopamine (DA) in nigro-striatal system was measured by a high performance liquid chromatography with electochemical detector (HPLC-ECD). The activities of superoxide dismutase 1(SOD1) and the content of malondialdehyde(MDA) were detected by the spectrophotometer. The injuries of nigraldopamine system and striatal oxidative stress related indexes were compared among mice at different ages. The expression of striatal oxidative stress related gene expression in 10-month mice was detected by PCR Array. Results Compared with control group, the levels of DA andSOD 1, the performance in the open field test all decreased in MPTP group at three ages, while the content of MDA in tissue remarkably increased (P<0.01). Moreover, the above changes in 10-month mice were more obvious than 3- and 6-month mice (P<0.05). Compared with control group, the PCR Array results of MPTP group showed that COX-2 was up-regulated, while glutathione peroxidase 3, 6 and 8, lactoperoxidase, nucleoredoxin, myoglobin, neuroglobin, peroxiredoxin 1, eosinophil peroxidase were all down-regulated (fold change>2). Conclusion Aging plays an important role in nigro-striatal system injury of PD model. Up- or down- regulation of oxidative stress related genes may participate in the early phase of PD.

Key words: Oxidative stress, Gene differential expression, Parkinson’s disease, Aging, Senescence-accelerated mouse prone 8

CLC Number: 

  • R741
[1] Van Den Eeden S K, Tanner C M, Bernstein A L, et al. Incidence of Parkinson's disease:variation by age, gender, and race/ethnicity[J]. Am J Epidemiol, 2003, 157(11):1015-1022.
[2] Sowell R A, Owen J B, Butterfield D A. Proteomics in animal models of Alzheimer's and Parkinson's diseases[J]. Ageing ResRev, 2009, 8(1):1-17.
[3] Zhang L, Li Q, Wolff L T, et al. Changes of brain activity in the aged SAMP mouse[J]. Biogerontology, 2007, 8(2):81-88.
[4] Colas D, Gharib A, Bezin L, et al. Regional age-related changes in neuronal nitric oxide synthase (nNOS), messenger RNA levels and activity in SAMP8 brain[J]. BMC Neurosci, 2006, 7:81.
[5] Luchtman D W, Shao D, Song C. Behavior, neurotransmitters and inflammation in three regimens of the MPTP mouse model of Parkinson's disease[J]. Physiol Behav, 2009, 98(1-2):130-138.
[6] Yamada M, Chiba T, Sasabe J, et al. Nasal Colivelin treatment ameliorates memoryimpairment related to Alzheimer's disease[J]. Neuropsychopharmacology, 2008, 33(8):2020-2032.
[7] 刘萍,罗本燕. 早期帕金森病进展危险因素研究[J]. 中国实用内科杂志, 2013, 33(7):548-551. LIU Ping, LUO Benyan. Risk factors for the progression of early stage Parkinson's disease[J].Chinese Journal of Practical Internal Medicine, 2013, 33(7):548-551.
[8] Yokoyama H, Kuroiwa H, Yano R, et al. Targeting reactive oxygen species, reactive nitrogen species and inflammation in MPTP neurotoxicity and Parkinson's disease[J]. Neurol Sci, 2008, 29(5):293-301.
[9] Sanders L H, Greenamyre J T. Oxidative damage to macromolecules in human Parkinson disease and the rotenone model[J]. Free Radic Biol Med, 2013, 62:111-120. doi:10.1016/j.freeradbiomed.
[10] Liu J, Wang M W, Gu P, et al. Microglial activation and age-related dopaminergic neurodegeneration in MPTP-treated SAMP8 mice[J]. Brain Res, 2010, 1345:213-220. doi:10.1016/j.brainres.2010.05.043.
[11] Nomura Y, Wang B X, Qi S B, et al. Biochemical changes related to aging in the senescence-accelerated mouse[J]. Exp. Gerontol, 1989, 24(1):49-55.
[12] Farr S A, Poon H F, Dogrukol-Ak D, et al. The antioxidants alpha-lipoic acid and N-acetylcysteine reverse memory impairment and brain oxidative stress in aged SAMP8 mice[J]. J Neurochem, 2003, 84(5):1173-1183.
[13] Periquet M, Fulga T, Myllykangas L, et al. Aggregated alpha-synuclein mediates dopaminergic neurotoxicity in vivo[J]. J Neurosci, 2007, 27(12):3338-3346.
[14] Wang T, Pei Z, Zhang W, et al. MPP+-induced COX2 activation and subsequent dopaminer gicneurodegeneration[J]. FASEB J, 2005, 19(9):1134-1136.
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