JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 1-5.doi: 10.6040/j.issn.1671-7554.0.2014.271

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RNAi-mediated silencing of CD147 inhibits the proliferation and invasion of leukemic cells SHI-1

TU Yan1,2, LI Zhenjiang1, TANG Aiping1, FEI Yan1, LI Huihui1, LI Jian1, HE Wenfeng1   

  1. 1. Department of Hematology, Second Hospital Affiliated to Nanchang University, Key Laboratory of Hematology in Jiangxi Province, Nanchang 330006, Jiangxi, China;
    2. Department of Hematology, Jinhua Hospital of Zhejiang University, Jinhua 321000, Zhejiang, China
  • Received:2014-04-14 Revised:2014-09-05 Online:2014-09-10 Published:2014-09-10
  • Contact: 李振江。E-mail:lzjdgh@163.com E-mail:lzjdgh@163.com

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Abstract: Objective To construct lentivirus vector for RNA interference (RNAi) targeting extracellular matrix metalloproteinase inducer (CD147) and to investigate the role of CD147 gene on the proliferation and infiltration of a human monocytic leukemic cell line SHI-1. Methods The complementary shRNA targeting CD147 was synthesized, and connected with pGCSIL-GFP vector. The 293T cells were transfected with recombined viral vector, packing plasmid pHelper 1.0 and pHelper 2.0 using Lipfectamine 2000. Then the recombinant lentiviruses were collected and infected the SHI-1 cells. The expressions of CD147, MMP-2 and MMP-9 in SHI-1 cells were detected by real time PCR. The protein of CD147 was detected by Western blotting. The capability of proliferation and infiltration of SHI-1 cell were examined by MTT and trans-matrigel invasion assay co-cultured with leukemia BMSCs in vitro. Results The lentivirus containing the CD147 shRNA was constructed successfully. The titer of recombined virus was 1×109 TU/mL. The mRNA and protein of CD147 in SHI-1/CD147i cells decreased by 86.7% and 91% respectively after the SHI-1 cells were infected by the lentivirus containing the CD147 siRNA. The proliferation capability of SHI-1/CD147i cells significantly decreased compared with that of SHI-1 and SHI-1/NC cells. The mRNA expressions of MMP-2 and MMP-9 in SHI-1/CD147i cells were significantly lower than that in SHI-1/NC and SHI-1 cells. The SHI-1/CD147i cells demonstrated significantly lower invasion rate than SHI-1 cells and SHI-1/NC cells when co-cultured with BMSCs. Conclusion CD147 plays important roles in the leukemia cell proliferation and infiltration. CD147 should be a potential target for the treatment of acute leukemia.

Key words: matrix metalloproteinase, Extracellular matrix metalloproteinase inducer, RNA interference, Cell invasion, Acute monocytic leukemia cell line, Cell proliferation

CLC Number: 

  • R733.7
[1] Stenzinger A, Wittschieber D, von W M, et al. High extracellular matrix metalloproteinase inducer/CD147 expression is strongly and independently associated with poor prognosis in colorectal cancer[J]. Human pathology, 2012, 43 (9): 1471-1481.
[2] Zeng W, Su J, Wu L, et al. CD147 promotes melanoma progression through hypoxia-induced MMP2 activation[J]. Curr Mol Med, 2014, 14(1): 163-173.
[3] Li Z J, Chen Z X, Cen J N, et al. Direct contact with bone marrow stromal cells promote the invasions of SHI-1 leukemia cells[J]. Chin Med J, 2013, 126 (14): 2731-2735.
[4] Froehlich H, Fellmann M, Sueltmann H, et al. Large scale statistical inference of signaling pathways from RNAi and microarray data[J]. BMC Bioinformatics, 2007, 8: 386-400.
[5] Ma Y, Chan C Y, He M L. RNA interference and antiviral therapy[J]. World J Gastroenterol, 2007, 13(39): 5169-5179.
[6] Jazag A, Ijichi H, Kanai F, et al. Smad4 silencing in pancreatic cancer cell lines using stable RNA interference and gene expression profiles induced by transforming growth factor-β[J]. Oncogene, 2005, 24(4): 662-671.
[7] Imamura T, Kanai F, Kawakami T, et al. Proteomic analysis of t heTGF-beta signaling pathway in pancreatic carcinoma cells using stable RNA interference to silence Smad4 expression[J]. Biochem Biophys Res Commun, 2004, 318 (1): 289-296.
[8] 黄灵芝, 赵敬湘, 刘淑静, 等. CD147 对白血病细胞U937生长和肿瘤形成的影响[J]. 生物技术通讯. 2008, 19(2): 203-206. HUANG Lingzhi, ZHAO Jingxiang, LIU Shujing, et al. The effect of CD147 on growth and tumorigenicity of leukemic U937 cells[J]. Letters in Biotechnology. 2008, 19(2): 203-206.
[9] Nabeshima K, Suzumiya J, Nagano M, et al. Emmprin, a cell surface inducer of matrix metalloproteinases (MMPs), is expressed in T-cell lymphomas[J]. J Pathol, 2004, 202(3): 341-351.
[10] Fu J F, Fu J F, Chen X Q, et al. CD147 and VEGF co-expression predicts prognosis in patients with acute myeloid leukemia[J]. Jpn J Clin Oncol, 2010, 40(11): 1046-1052.
[11] Beesley A H, Cummings A J, Freitas, et al. The gene expression signature of relapse in paediatric acute lymphoblastic leukaemia: implications for mechanisms of therapy failure[J]. Br J Haematol, 2005, 131(4): 447-456.
[12] Thorn C S, Noack F, Feller A C, et al. Application of newly developed tissue-arrays to study EMMPRIN (CD147) expression in malignant Non-Hodgkin lymphoma[J]. Cancer Genomics Proteomics, 2004, 1(1): 45-52.
[13] Arendt B K, Walters D K, Wu X, et al. Increased expression of extracellular matrix metalloproteinase inducer (CD147) in multiple myeloma: role in regulation of myeloma cell proliferation[J]. Leukemia, 2012, 26(10): 2286-2296.
[14] Xu J, Shen Z Y, Chen X G, et al. A randomized controlled trial of Licartin for preventing hepatoma recurrence after liver transplantation[J]. Hepatology, 2007, 45(2): 269-276.
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