JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (10): 9-14.doi: 10.6040/j.issn.1671-7554.0.2014.313

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Early effect of shear stress on the apoptosis of pulmonary microvascular endothelial cells

DU Qingxia1,2, LI Fuhai1, ZHAO Cuifen1, KONG Qingyu1, HUANG Xiaoyang1, XIA Wei1   

  1. 1. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Jinan Pediatric Hospital, Jinan 250022, Shandong, China
  • Received:2014-05-15 Revised:2014-09-18 Online:2014-10-10 Published:2014-10-10

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Abstract: Objective To observe the early effect of shear stress on the apoptosis of pulmonary microvascular endothelial cells (PMVECs) and the expression of anti-apoptotic gene BCL-2, and to elucidate the early pathogenesis of pulmonary hypertension induced by high pulmonary blood flow. Methods Self-made perfusion system was used to exert shear stress on rat PMVECs. The Annexin V/PI staining was applied to observe the apoptosis of different groups. Real-time PCR and Western blotting were performed to measure the relative expressions of BCL-2 in the mRNA and protein levels respectively. Results Flow cytometry analysis showed that the shear stress could induce the apoptosis of the PMVECs, which would increase with the extending of the acting time. The relative expression of BCL-2 in the 0.5 h group was lower than that in the control group, and the expression would increase as the acting time extended. There was significant difference between the groups. Conclusion The shear stress can promote the apoptosis of the PMVECs and alter the expression of anti-apoptotic gene BCL-2.

Key words: Shear stress, Pulmonary microvascular endothelial cells, Hypertension, pulmonary, Apoptosis

CLC Number: 

  • R725.4
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