慢病毒载体靶向携带survivin-siRNA对恶性黑色素瘤A375细胞的影响

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2013, Vol. 51 ›› Issue (5): 54-57.

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The effect of the survivin-siRNA targeting lentivirus vector on human malignant melanoma cell line A375

ZHU Shou-rong1， ZHANG Rui2， CAO Yong-qian1， FENG Zhang1， WANG Yi-bing1

1. Burn and Plastic Surgery， Provincial Hospital Affiliated to Shandong University， Jinan 250021， China；
2. Department of Pathology and Pathophysiology， Shandong Medical Collegy， Jinan 250002， China

Received:2012-08-09 Online:2013-05-10 Published:2013-05-10

Abstract

Abstract:

Objective To observe the effect of the RNA interference (RNAi) targeting survivin on human malignant melanoma cell line A375. Methods The human malignant melanoma cell line A375 was divided into there groups: the survivin-siRNA LV group, the NC-GFP-LV group and the control group. After designing and constructing the survivin-siRNA lentivirus vector, transfect it into these there groups of cells. The expression of survivin mRNA and protein were detected by real time-PCR and Western blot respectively. Results The survivin-siRNA lentivirus vector was designed and constructed successfully. After the transfection, the expressions of survivin mRNA and protein were significantly lower in the survivin-siRNA LV group than those in the control group(P<0.05), with the inhibition ratio of 60% and 87.38%. Conclusion The shRNA targeting survivin can down-regulate the expression of survivin gene in melanoma A375 cells.

Key words: Malignant melanoma； Survivin gene； RNA interference； Lentivirus vector

CLC Number:

R730.5

ZHU Shou-rong1， ZHANG Rui2， CAO Yong-qian1， FENG Zhang1， WANG Yi-bing1. The effect of the survivin-siRNA targeting lentivirus vector on human malignant melanoma cell line A375[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 54-57.