Abstract:

Objective   To investigate the biocompatibility and biodegradation of a novel porcine acellular dermal matrix hydroxyapatited (HA-PADM) scaffold. Methods   The biocompatibility of three kinds of scaffolds (HA-PADM, PADM, Gelfoam) in vivo was assessed by implanting them into the sacrospinal muscle of 12 New Zealand white rabbits and extracting them after 7， 14， 21， and 28 days. Haematoxylin Eosin (HE) staining was used to evaluate the biocompatibility of the scaffolds. Blood routine examinations were carried out before the operation and 2, 4 days after the operation. The biodegradation patterns of the scaffolds were evaluated in vitro by weight assessment at different time points after cultivation in the cell-culture medium (DMEM). Results   There were no statistically differences in the results of blood routine examinations of all animals (P>0.05). Around the embedded scaffolds there was no formation of abscess and no tissue necrosis of the muscle. The local tissue reaction was abacterial inflammatory response. The biodegradation of the scaffolds met the needs of periodontal tissue regeneration. Conclusion   The biocompatibility of HA-PADM is favorable and the biodegradation time is proper. HA-PADM scaffold might be a promising scaffold for periodontal tissue regeneration.

Key words: Scaffold; Biocompatibility; Biodegradation; Periodontal tissue regeneration