Abstract:

Objective         To explore differential expression of microRNA(miRNA) profile in endometrioid endometrial adenocarcinoma(EEC) compared with the normal endometrium and to explore the role of these miRNAs in EEC. MethodsmiRNA microarray was used to detect differential expressions of miRNAs, and real-time-PCR was used to verify the results. Then targets of these miRNAs were predicted through Web databases. Prediction analysis for microarray(PAM)was used to predict a group of miRNA biomarkers, which could be used to identify different differentiation samples of EEC. Results      18 miRNAs were obviously up-regulated and 31 obviously down-regulated in EEC. 52 genes like PTEN and FOX were predicted as targets of these differently regulated miRNAs. mir-200c and other 8 miRNAs were considered as members of the biomarker group. Conclusions      Several miRNAs and predicted targets of special research value are picked out. They provide valid evidence for further study of EEC.

Key words: MicroRNA; Endometrioid endometrial adenocarcinoma; miRNA microarray; Target gene; Prediction analysis for microarray