JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (8): 70-73.

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TGF-β1-related model peptides isolated from a phage  display 12-mer peptide library

LIU Zhenzhong1, JIANG Duyin1,2, CAI Jinglong3, ZONG Xianlei3, ZHANG Jixun1, SHAN Fei1,2, WANG Wenting1,2, WANG Wei1,2   

  1. 1. Department of Plastic Surgery, The Second Hospital of Shandong University, Jinan 250033, China;
    2. Institute of Tissue Engineering, The Second Hospital of Shandong University, Jinan 250033, China;
    3 Sixteenth Department  of Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100144, China
  • Received:2011-03-29 Online:2011-08-10 Published:2011-08-10

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Abstract:

Objective      To harvest TGF-β1 model peptides from a phage display 12-mer peptide library. Methods      A phage display 12-mer peptide library was screened 4 times by the monoclonal anti-human TGF-β1. Monoclonal phages were randomly selected by ELISA. DNA extracted from phages with better binding activity was sequenced and analyzed. Results      After 4 times of screening, abundant specific phage model peptides were harvested. The results of ELISA showed that some phage model peptides had a fine binding activity. A total of 10 sequences related to promoting or inhibiting fibroblast cell proliferation were recognized. Conclusion      TGF-β1-related phage model peptides can be harvested from a phage display 12-mer peptide library, and some phage model peptides may inhibit keloid fibroblast proliferation.

Key words: Phage display 12-mer peptide library; TGF-β1 phage model peptides; Biological screening; Sequencing

CLC Number: 

  • R622
[1] . hFGF7 related modeling peptide screening with phage random twelve peptide library [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(11): 46-49.
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