JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (8): 153-.

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Molecular typing of Listeria monocytogenes isolated from foods in  Shandong Province by PulsedField Electrophoresis

JIA Jing1, BI Zhenwang2, CHEN Yuzhen2, HOU Peibin2, ZHANG Ming2, SHAO Kun2, BI Zhenqiang1，2   

  1. 1. Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China;
    2. Shandong Center for Disease Control and Prevention; Shandong Key Laboratory of Infectious Diseases Prevention and
    Control; Institute of Preventive Medicine, Shandong University, Jinan 250014, China
  • Received:2011-04-11 Online:2011-08-10 Published:2011-08-10

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Abstract:

Objective     To investigate the genetic correlation and epidemiological significance of different genotypes of Listeria monocytogenes (LM) isolated from foods in Shandong Province during 2007-2009. Methods     100 strains were genotyped by pulsedfield gel electrophoresis (PFGE), and the images were processed by cluster analysis using computer BioNumerics software. Results     Digestion of DNA with AscⅠ yielded 10 to 15 bands. The 100 Shandong Listeria monocytogenes were subtyped into 31 PFGE types, of which the type 4 was the majority. Conclusions     In Shandong Province, LM strains were from different clones, but a number of LM strains were closely related; PFGE is a useful method in analyzing homology of LM, and it is of great significance in molecular epidemiological studies of LM.

Key words: Listeria monocytogenes; Pulsed-field gel electrophoresis; Molecular typing

CLC Number: 

  • TS201.3
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