Abstract:

Objective    To explore the inhibitory effect of scutelalrin on methylcyclopentadienyl manganese tricarbonyl (MMT)-induced apoptosis in SK-N-SH cells and its possible mechanism. Methods    SK-N-SH cells were randomly grouped into 5 groups: the normal group, the MMT induction group, the 1.2×10-1mg/L scutellarin group， the 3.0×10-2mg/L scutellarin group and  the 7.5×10-3mg/L scutellarin group. After incubation with scutelalrin for 2h， SKNSH cells were induced by 1mmol/L of MMT for 24h. Then viability of cells was determined the MTT assay. The production of reactive oxygen species (ROS) and  malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were determined by a spectrophotometer. The release of adenosine triphosphate(ATP) and activity of Caspase-3 were measured by chemiluminescence. Results     After incubation with scutelalrin, the viability of cells markedly ascended（P＜0.05）. The production of ROS and MDA was inhibited （P＜0.01）, while the activity of SOD was increased（P＜0.05）. The release of ATP partly resumed（P＜0.05）, while the activity of Caspase-3 was significantly inhibited（P＜0.01）. Conclusion    Scutelalrin can inhibit apoptosis of SK-N-SH cells induced by MMT through anti-oxidation in this process.

Key words: Scutelalrin; Methylcyclopentadienyl manganese tricarbonyl; Neuron; Apoptosis