Abstract:

Objective    To measure blood and tissue fluid concentrations of levofloxacin (LVFX) in rabbits and calculate the drug and pharmacokinetic parameters after intravenous injection， and then provide the basis for the transition of common pathogens such as Pseudomonas aeruginosa in vitro studies to in vivo. Methods    The rabbit cage models were established, and then the rabbit blood and  tissue fluid concentrations of LVFX at different times were measured by high performance liquid chromatography(HPLC). The pharmacokinetic parameters were calculated. Results    After 10mg/kg LVFX was intravenously injected in rabbits，the peak concentration (Cmax ) of LVFX in blood and tissue fluid was (8.27±0. 9) and (2.48 ±0. 3)mg／L ; the half-life ( T1/ 2β) of various concentrations in blood and tissue fluid was (1.06±0.1) and (3.63±1.3)h; the apparent volume of distribution was （1.55±0.3）and（4.89±2.1）L/kg, respectively. Conclusion    LVFX shows good penetration when administered by intravenous injection in the tissue fluid. Both the long half-life of LVFX and small fluctuation of drug concentration could be helpful to maintain therapeutic levels in tissue fluid.

Key words:  Ofloxacin; Tissue distribution; Pharmacokinetics; Chromatography, high pressure liquid