JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2010, Vol. 48 ›› Issue (2): 44-47.

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Expression and significance of caspase-3, bax and bcl-2 in the hypothalamus of rats after experimental subarachnoid hemorrhage

LIU Dianwei1, JIANG Yong1, HAN Xiangyang2   

  1. 1. Department of Neurosurgery, Jinan Central Hospital Affiliated to Shandong University, School of Medicine, Shandong University, Jinan 250013, China;
    2. Healthcare department, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China
  • Received:2009-10-14 Online:2010-02-16 Published:2010-02-16

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Abstract:

Objective  To investigate changes of apoptosis-related molecules at various time points after subarachnoid hemorrhage (SAH) in the hypothalamus of rats. Methods   SAH models were made by the double hemorrhage method. Gene expressions of caspase-3, bax, and bcl-2 at different time points after SAH were detected using real-time PCR. In addition, western blot and Nissl staining were separately employed to further confirm protein and morphological changes at 48h after SAH. Results  Gene expressions of caspase-3, bax and bcl-2 which peaked at 48h after surgery had a significant difference compared with the control group(P＜0.05). Also, compared with the control group, proteins of caspase-3, bax, and bcl-2 were significantly increased in the experimental group at 48h(P＜0.05). Conclusion  The activated apoptotic and anti-apoptotic pathway in the hypothalamus of rats which suffered SAH may be related to the disorder of hormone secretion.

Key words: Subarachnoid hemorrhage; Hypothalamus; Apoptotic factor

CLC Number: 

  • R651.1
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