Abstract:

Objective To compare the ability of type Ⅰand type Ⅱ endometrium cancer cells for resisting oxidative stress and investigate its related mechanism Method  ①  TypeⅠand type Ⅱ endometrium cancer cells were challenged by H₂O₂, and cell proliferation was determined by the MTT assay. ② TypeⅠor type Ⅱ endometrium cancer cells were treated with 120μmol H₂O₂ or 800?μmol H₂O₂, and cellular ROS, GSH, and GSSG levels and SOD, CAT and GPx activities were determined.  Results Without H₂O₂, typeⅠ endometriumcancer cells, compared with the type Ⅱ endometrium cancer cells, had a lower ROS level and CAT activity and a higher GSH level, GSH/GSSG ratio, and SOD and GPx activities. Under oxidative stress, the ROS level increased in both typeⅠand type Ⅱ endometrium cancer cells. GSH level, GSH/GSSG ratio, and SOD and CAT activities were decreased in type Ⅰ endometrium cancer cells while GSH and GSSG levels, GSH/GSSG ratio, and SOD, CAT and GPx activities increased in type Ⅱ endometrium cancer cells.  Conclusion  Oxidative stress is higher in typeⅡendometrium cancer cells than in typeⅠendometrium cancer cells. The oxidationreductions ystem and anti-oxidase system in type Ⅱ endometrium cancer cells can effectively respond to oxidative stress, and cells can effectively resist oxidative stress.

Key words: Endometrium neoplasms; Reactive oxygen species; Glutathione; Antioxidase