JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2009, Vol. 47 ›› Issue (5): 50-53.

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Construction of nervous specific promoter PDGF-EGFPvector and  identification of tissue specificity

 REN Kai, DING Yan, CUI Yun-Yan, YANG Ling-Ling, ZHANG Jing, CUI Xing   

  1. 1. Department of Biochemistry, Xi′an Medical University, Xi′an 710021, China; 2. Department of Biochemistry and Molecular Biology, Life and Science School, Xi′an Jiaotong University, Xi′an 710049, China; 3. Jinan Central Hospital, Jinan 250013, China; 4. Department of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China
  • Received:2008-10-10 Online:2009-06-16 Published:2009-05-16

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Abstract:

Objective To construct an expression vector of enhanced green fluorescent protein (EGFP) containing nervous tissue-specific promoter-PDGF.  Methods  The PDGF-promoter sequence was amplified by PCR and the template was the genome of human blood cells. The PCR products were cloned into the pEGFP-1 vector and then pP DGF-EGFP vector was constructed. The vector was transfected into human neuroblastoma cells, SK-N-SH and four kinds of non-nervous recourse cells by lipofectamine. Expression of EGFP of transfected cells and tissue specificity of the vector were determined by transfection.  Results The vector of pPDGF-EGFP was successfully constructed. After a transfection experiment, the EGFP was highly expressed in SK-N-SH cells and scarcely expressed in the other four cells.  Conclusion  The vector of pPDGF-EGFP has a high nervous tissue specificity. It lays a foundation for studying target gene therapy of nervous system diseases.

Key words: latelet-derived growth factor; Nervous tissue-specific promoter; Cell lines

CLC Number: 

  • R741.02
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