Abstract:

Objectives To synthesize and characterize biotinylated chitosan.  Methods  A new targeting carried material (biotinylated chitosan) was synthesized by reaction of the amino group of chitosan and carboxy group of biotin under 4-Dimethylaminopyridine and N, N′-dicyclohexylcarbodiimide catalyst conditions after being depurated by  dialysis methods and was lyophilized. The chemical structure of the synthesized biotinylated chitosan was characterized by the Fourier transform ation  infrared spectrometer (FTIR), nuclear magnetic resonance (1HNMR), and differential scanning calorimeter (DSC) methods  Results A white power solid biotinylated chitosan was obtained after refinement, and figures of its FTIR,1HNMR and DSC were different from its monomer. The substitution value was 42.28% and the yield was14.95%.  Conclusion  Biotin and chitosan are chemically bonded through an amide linkage so that the Biotinylated chitosan is successfully synthesized with simpleprocesses and low cost.

Key words: Biotinylated; Chitosan; Synthesize; Characterization;Targeting carried material