JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2009, Vol. 47 ›› Issue (12): 58-60.

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Effect of tamoxifen on the Smad 7 expression and proliferation of human hepatoellular carcinoma  HepG2 cells

FAN Yifeng 1 ,　WANG Wenqi 2 ,　BAI Wenkun 2 ,　SHI Changwen 3   

  1. 1. School of Medicine, Shandong University, Jinan 250012, China;
    2. Department of Gastroenterology, Shandong Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China;
    3. Shandong  Provincial Key Lab of  Surgery, Jinan 250014, China
  • Received:2009-07-10 Online:2009-12-16 Published:2009-12-16

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Abstract:

To study the effect of tamoxifen  on proliferation of  human hepatoellular carcinoma HepG2 cells. MethodsThe HepG2 cells were treated with tamoxifen at concentrations of  0,7.5，15 and 30?μmol/L. The cells were further cultured for 24，48 and 72?h．The suppression rate of HepG2 cells was determined with MTT. The Smad7 expressive rate of HepG2 cells was examined by immunohistochemistry．ResultsTamoxifen inhibited the proliferation of HepG2 cells in a dose and time dependent manner．Tamoxifen suppressed expression of Smad7 of HepG2 cells also in a dose and time dependent manner．ConclusionsTamoxifen may suppress human hepatocelular carcinoma HepG2 cell proliferation by downregulating Smad 7 expressions．

Key words:  Carcinoma, Hepatocelular; Tamoxifen; Smad 7

CLC Number: 

  • R735.7
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