Abstract:

To explore expression of SIRT1 in delaying axonal degeneration by NAD. MethodsAfter establishing an axonal degeneration model of DRG neurons induced by vincristine, transmission electron microscopy, RTPCR, and Western blotting analysis were used to observe expressions of mRNA and protein of SIRT1 in the normal group, injury group and protection group. ResultsThe axons were swollen and disrupted in the distal end of axon at 4?h in vitro culture of the injury group. Obvious protective effects were detected in the experimental groups containing NAD, and the end of axon showed light degeneration at 12?h in culture. Transmission electron microscopy showed that the smooth endoplasmic reticulum of the axons dilated and formed vacuoles in the injury group. Also, the normal neurofilament axonal metastructure decreased and turned to granular fragments. RTPCR examination showed that mRNA 1ConclusionNAD plays an important role in preventing axonal degeneration, and SIRT1 probably participates in preventing axonal degeneration of NAD.

Key words: NAD； Regulatory sequences, nucleic acid; Neurites