JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• 无栏目 • Previous Articles     Next Articles

Mesenchymal stem cells combined with rapamycin immunoregulates
the spleniclymphocytes in allogenic mice

YIN Yujun1, LI Jing2, TANG Yu2, YOU Haiyan3, ZHU Wei4,　XU Wengrong4, JIAO Zhijun3
   

  1. （1. Department of Dermatology, 2. Department of Rheumatology, 3. Department Laboratory, Affiliated Hospital of Jiangsu University,
    Zhenjiang 212001, Jiangsu, China； 4. School of Medical Technology, Jiangsu University, Zhenjiang 212013, Jiangsu, China）
  • Received:1900-01-01 Revised:1900-01-01 Published:2009-04-16
  • Contact: LI Jing

PDF (PC)

167

Abstract: To investigate the immunoregulatory effects of mesenchymal stem cells(MSCs) combined with rapamycin on T and B cells in vitro and its potential mechanism. MethodsMSCs were isolated and cultivated from the bone marrow of 56 weekold BALB/c mice in vitro. The purity was detected through surface markers by flow cytometry(FCM). Splenic lymphocytes were isolated by EZSep(tm) Mouse 1X. Under ConA or LPS stimulation, lymphocytes were treated with MSCs and/or rapamycin. The proliferation was assessed by MTT coloremetry. FCM was used to analyze the apoptosis and surface markersCD69， CD28 and CD86. mRNA expressions of cytokines were detected by realtime quantitative PCR. ResultsBoth MSCs and MSCs combined with rapamycin could significantly inhibit proliferation of lymphocytes and the apoptosis of T cells. However, the inhibitory effect of the latter was more obvious than that of the former (P＜0.01). Both MSCs and MSCs combined with rapamycin could significantly increase expression of interferon (IFN)γ mRNA but decrease expression of interleukin (IL)10. No differences of CD69, CD28 and CD86 expressions were observed among all groups. ConclusionMSCs combined with rapamycin could downmodulate the function of T and B cells, and the potential mechanism may be related to its effect on the proliferation，apoptosis and mRNA expressions of IFNγ and IL10.

Key words: Mesenchymal stem cells, Rapamycin, Lymphocytes, Immunoregulatory

CLC Number: 

  • R392
[1] CHEN Yang, FENG Ying, LU Xiao, ZHENG Rong. Galectin-3 promotes autophagy and polarization of macrophages via the PI3K/Akt/mTOR pathway [J]. Journal of Shandong University (Health Sciences), 2026, 64(4): 14-22.
[2] ZHAO Canbin, SHAO Jiang, GUAN Donghui, QIN Ying, DING Qiang, GUO Liang, WANG Weiwei, CHEN Wei, YAN Xiaolong, ZENG Ping. Mechanism of luteolin regulating Wnt/β-catenin signal pathway in inflammatory microenvironment to promote chondrogenic differentiation of bone marrow mesenchymal stem cells [J]. Journal of Shandong University (Health Sciences), 2025, 63(7): 11-22.
[3] LIU Haixia, HUANGFU Shasha, SANG Xiaoyu, CUI Dongqing, BI Jianzhong, WANG Ping. Effects of mesenchymal stem cells on ferroptosis in experimental autoimmune encephalomyelitis mice [J]. Journal of Shandong University (Health Sciences), 2024, 62(6): 1-8.
[4] SHEN Feifei, LI Dong, LIU Linghong, JU Xiuli. Umbilical cord mesenchymal stem cells sheets treated by small intestinal submucosa promote wound healing [J]. Journal of Shandong University (Health Sciences), 2024, 62(4): 68-77.
[5] YAN Xiaolong, QIN Ying, SHAO Jiang, CHEN Dongfeng, GUAN Donghui, ZHAO Canbin. Mechanism of curcumin regulating bone formation via the Wnt/β-catenin signaling pathway [J]. Journal of Shandong University (Health Sciences), 2024, 62(10): 76-86.
[6] HE Jing, YAN Rugen, WU Zhihong, LI Changzhong. Effects of Xiaozheng Yiai decoction on the proliferation and migration of ovarian cancer SKOV3 cells [J]. Journal of Shandong University (Health Sciences), 2023, 61(5): 1-10.
[7] XIE Xiaoli, QIU Yu, WANG Lijuan. IFN-γ promots the proliferation of NHL by up-regulating PD-L1 via PI3K/Akt/mTOR pathway [J]. Journal of Shandong University (Health Sciences), 2023, 61(5): 11-19.
[8] WANG Cheng, ZHANG Shuhui, YAN Na, MENG Linlin, CHEN Liangzhi, LIU Qinghua. Mechanism of umbilical cord mesenchymal stem cells treating bleomycin-induced pulmonary interstitial fibrosis in mice [J]. Journal of Shandong University (Health Sciences), 2023, 61(10): 17-22.
[9] XIANG Yujiao, LIU Qiang, LIU Lu, SHI Yan. Mechanism of abnormal immune response of dendritic cells in immune thrombocytopenia [J]. Journal of Shandong University (Health Sciences), 2022, 60(7): 89-97.
[10] JU Xiuli. Potential mechanism and research progress of mesenchymal stem cells in the treatment of 2019 novel coronavirus pneumonia [J]. Journal of Shandong University (Health Sciences), 2020, 58(3): 32-37.
[11] XU Yanyan, YAN Bei, WANG Rui, WANG Hongyan. Menstrual blood mesenchymal stem cells improve premature ovarian failure in mice via IGF-1 signaling [J]. Journal of Shandong University (Health Sciences), 2020, 58(2): 13-20.
[12] ZHANG Hairong, ZHANG Xiaohong, WANG Chaoqun. A metabonomic study on mTOR pathway regulating radiosensitivity of ECA109 cells [J]. Journal of Shandong University (Health Sciences), 2020, 58(1): 6-12.
[13] ZHAO Hongjian, ZHOU Jijun, SU Qingliang, ZHAO Shuo, LI Yuming. Correlation between human gastric cancer mesenchymal stem cells and tumor progression in SGC-7901 tumor-bearing mice [J]. Journal of Shandong University (Health Sciences), 2019, 57(9): 69-73.
[14] ZHANG Zhihui, LIU Yanguo, XIAO Fengqi, MA Daoxin, WANG Xiuwen. Effect of cisplatin on IL-6 expression in tumor mesenchymal stem cells and underlying mechanism of p38MAPK pathway [J]. Journal of Shandong University (Health Sciences), 2019, 57(3): 1-6.
[15] WANG Bo, XUE Jiang, LIU Aihong, ZHAI Ruirui, WANG Yibiao. Rapamycin improved pulmonary artery hypertension via modulating the phenotypes of macrophages [J]. Journal of Shandong University (Health Sciences), 2018, 56(4): 51-57.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd