Abstract: To investigate expressions of B7H4, CA125 and P53 in primary epithelial ovarian cancer (EOC) and to explore their relationship with prognosis. MethodsExpressions of B7H4, CA125 and P53 were determined by PV6000 twostep immunohistochemistry in 70 cases of EOC, 20 cases of normal ovarian tissues and 20 cases of benign ovarian tumors. ResultsThe positive rate of B7H4 in epithelial ovarian cancer was 88.5%(62/70), and it was significantly higher than that in benign ovarian epithelial tumors (45%) and normal ovarian tissues (0%) .The positive rate of CA125 in epithelial ovarian cancer was 77.1%, and it positively correlated with B7H4. The positive rate of P53 in epithelial ovarian cancer was 68.6% (48/70), and was significantly lower than B7H4 (P<0.05). Multivariate COX regression analysis showed that prognosis of epithelial ovarian cancer was correlated with the FIGO stage, residual tumor size, B7H4′s positive expression, cell differentiation, CA125′s positive expression, P53′s positive expression and lymph node metastasis. Single factor KaplanMeier analysis showed that the intensity the B7H4 positive expression was negatively correlated with the average survival time of patients and the cumulative fiveyear survival rates. ConclusionB7H4 overexpresses in epithelial ovarian cancer and positively correlates with CA125 and is higher than P53 expression. B7H4 is an independent risk factor of total life span of epithelial ovarian cancer patients. The stronger the B7H4 expression, the shorter the average life time and the lower the cumulative fiveyear survival rate.

Key words: Ovarian neoplasms, B7H4, Protein p53, Prognosis